We narrowed to 13,071 results for: lic

1. pCgCas9_recT

   Plasmid

   #153337

   Purpose

   Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniTurbo-V5_pRS415

   Plasmid

   #107168

   Purpose

   expresses V5-tagged miniTurbo in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Yeast

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEC2861 (pSpy1C-PgyrA_ffluc)

   Plasmid

   #218511

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PgyrA

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. C1(1-29)-TurboID-V5_pLX304

   Plasmid

   #107175

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Lentiviral

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lyso-GA

   Plasmid

   #209874

   Purpose

   Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP A
   

   Tags

   LAMP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-H-Luc_cyto

   Plasmid

   #113929

   Purpose

   Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. C1(1-29)-miniTurbo-V5_pCDNA3

   Plasmid

   #107174

   Purpose

   expresses V5-tagged miniTurbo on the mammalian ER membrane

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav-GA

   Plasmid

   #209880

   Purpose

   Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP A
   

   Tags

   CAVIN1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PM-GA

    Plasmid

    #209877

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Tags

    Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZac2.1 gfaABC1D-eGFP

    Plasmid

    #176861

    Purpose

    Expresses cytosolic eGFP in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    eGFP
    

    Use

    AAV

    Expression

    Bacterial

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

    Plasmid

    #209728

    Purpose

    Expresses cytosolic high affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-hfYFP

    Plasmid

    #186523

    Purpose

    Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

    Plasmid

    #126553

    Purpose

    Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    Thy1.2 and GFP

    Promoter

    HIV LTR

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBTet_NADP-Snifit-cyto

    Plasmid

    #113917

    Purpose

    Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PM-RA

    Plasmid

    #209878

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRANS_253

    Plasmid

    #91125

    Purpose

    Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. C1(1-29)-miniTurbo-V5_pLX304

    Plasmid

    #107176

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Lentiviral

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRT801

    Plasmid

    #68112

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cav-RA

    Plasmid

    #209881

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    CAVIN1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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