Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 11681 - 11700 of 12917 results

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pminiTol2-4kb wnt3 promoter-PMTmApple

Plasmid

#203794

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

Insert

PMT

Use

Zebrafish expression

Tags

mApple

Promoter

4kb Wnt3 promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 - circMAN2A1

Plasmid

#198837

Purpose

This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

Depositor

Stefan Stamm

Article

Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

Insert

circular mannosidase alpha class 2A member 1

Expression

Mammalian

Mutation

circular RNA composed by 2 exons

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2A-Kina

Plasmid

#202745

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108) : [GGSA]4GG :PIP4K2A (33-406) (PIP4K2A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2C

Plasmid

#202736

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2C (PIP4K2C, FKBP1A Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2CD280K

Plasmid

#202737

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2C (D278K) (PIP4K2C, FKBP1A Human)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pETM30-C9ORF78

Plasmid

#193367

Purpose

Expression of C9ORF78 in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

Depositor

Markus Wahl

Article

Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

Insert

C9ORF78 (C9orf78 Human)

Tags

His6-GST-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV W131G SPOP 1-374

Plasmid

#197019

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

W131G

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ3A-(HA)3

Plasmid

#198179

Purpose

Expression of HA-tagged AP-3 μ3A in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-3 μ3A

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 225

Promoter

CMV

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV E78K SPOP 1-374

Plasmid

#197018

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

E78K

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV E47K SPOP 1-374

Plasmid

#197017

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

E47K

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV W22R SPOP 1-374

Plasmid

#197016

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

W22R

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NH-HsNot3_607-753-NpM-HsNot2-344-540_U

Plasmid

#147723

Purpose

Bacterial Expression of HsNot3_607-753-NpM-HsNot2-344-540

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_607-753-NpM-HsNot2-344-540 (CNOT3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del344-380_T

Plasmid

#147629

Purpose

Mammalian Expression of DmNot2-del344-380

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del344-380 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del380-404_T

Plasmid

#147630

Purpose

Mammalian Expression of DmNot2-del380-404

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del380-404 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del409-432_T

Plasmid

#147631

Purpose

Mammalian Expression of DmNot2-del409-432

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del409-432 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot3-del607-634_T

Plasmid

#147632

Purpose

Mammalian Expression of HsNot3-del607-634

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-del607-634 (CNOT3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot3-del632-654_T

Plasmid

#147633

Purpose

Mammalian Expression of HsNot3-del632-654

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-del632-654 (CNOT3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC33_D

Plasmid

#146141

Purpose

Mammalian Expression of tubcDNA-PTC33

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC33

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC62_D

Plasmid

#146142

Purpose

Mammalian Expression of tubcDNA-PTC62

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC62

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-QQN

Plasmid

#193563

Purpose

Expresses human Clathrin Light Chain B mutant (residues 20-22 were substituted from EED to QQN) tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Changed from EED to QQN at residue 20-22

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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