We narrowed to 13,695 results for: crispr cas9

1. pIW082

   Plasmid

   #134136

   Purpose

   Retroviral expression of GFP-Ska1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CRISPR resistant, R236A

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIW088

   Plasmid

   #134135

   Purpose

   Retroviral expression of GFP-Ska1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CRISPR resistant, K223A K226A

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIW085

   Plasmid

   #134134

   Purpose

   Retroviral expression of GFP-Ska1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CRISPR resistant, K183A K184A

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA0057

   Plasmid

   #131784

   Purpose

   Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

   Depositor

   Frank Albert

   Article

   Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

   Insert

   20mer GFP cassette guide (gGFP) and 5' sgRNA
   

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

   Plasmid

   #217305

   Purpose

   sgRNA BFP + Puromycin

   Depositor

   Luke Gilbert

   Insert

   pU6-sgRNA, Ef1alpha-Puro-T2A-BFP
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   U6, Ef1alpha

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

   Plasmid

   #124537

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41 C55G

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

   Plasmid

   #124542

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41 C55G

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124543

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTK0_047

   Plasmid

   #123977

   Purpose

   Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   hCLYBL CAS9 Destination - HygroR
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124541

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124539

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124544

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124540

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124538

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. H513 px330.sgActin.UTR.2

    Plasmid

    #138176

    Purpose

    CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

    Depositor

    Wen Xue

    Article

    Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

    Insert

    sgActin (Actb Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX333-T2A-EGFP

    Plasmid

    #197417

    Purpose

    SpCas9-T2A-EGFP with dual sgRNA cloning backbones

    Depositor

    Yeh-Hsing Lao

    Article

    Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh; U6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS156

    Plasmid

    #182707

    Purpose

    CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

    Depositor

    Rebecca Shapiro

    Article

    Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pV1539

    Plasmid

    #111434

    Purpose

    Genedrive entry vector for Candida albicans

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PDG458 eSp(1.1) V3

    Plasmid

    #226964

    Purpose

    CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

    Plasmid

    #211817

    Purpose

    AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    N-terminus of SpCas9-ABE8e and tracrRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter and U6 promoter

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge