We narrowed to 13,668 results for: cdna

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2x3APWWP_K299E_D333A-IFP2.0C

Plasmid

#215753

Purpose

Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2x3APWWP_K299E_D333A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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NSP2-Flag

Plasmid

#210344

Purpose

Expresses SARS-CoV-2 NSP2 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP2 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(3G)-mCherry

Plasmid

#206945

Purpose

Mammalian expression of heterobifunctional SasG linkers (3G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (3G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TLNRD1_35-345_eGFP

Plasmid

#208881

Purpose

Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 (TLNRD1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acid sequence 35-345

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TLNRD1_5H_eGFP

Plasmid

#208861

Purpose

Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 5 helix bundle (TLNRD1 Human)

Expression

Mammalian

Mutation

Deleted amino acid 142-273

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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SEC61B-2Strep

Plasmid

#210375

Purpose

Expresses SEC61B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SEC61B (SEC61B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TMEM106B-2Strep

Plasmid

#210360

Purpose

Expresses TMEM106B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TMEM106B (TMEM106B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f L27A

Plasmid

#204140

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Leucine 27 changed to Alanine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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ALIX(P801G)-mNeonGreen

Plasmid

#191192

Purpose

Full-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (P801G) (PDCD6IP Human, Synthetic)

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Pro 801 mutated to Gly

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(7G)-mCherry

Plasmid

#206949

Purpose

Mammalian expression of heterobifunctional SasG linkers (7G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (7G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(6G)-mCherry

Plasmid

#206948

Purpose

Mammalian expression of heterobifunctional SasG linkers (6G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (6G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(5G)-mCherry

Plasmid

#206947

Purpose

Mammalian expression of heterobifunctional SasG linkers (5G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (5G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(4G)-mCherry

Plasmid

#206946

Purpose

Mammalian expression of heterobifunctional SasG linkers (4G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (4G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(2G)-mCherry

Plasmid

#206944

Purpose

Mammalian expression of heterobifunctional SasG linkers (2G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (2G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMextli_577-620_AB

Plasmid

#148337

Purpose

Insect Expression of DmMextli_577-520

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-520 (mxt Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMextli_577-640_AB

Plasmid

#148338

Purpose

Insect Expression of DmMextli_577-640

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640 (mxt Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmMextli_577-620_AB

Plasmid

#148355

Purpose

Bacterial Expression of DmMextli_577-620

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-620 (mxt Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmMextli_577-640_AB

Plasmid

#148360

Purpose

Bacterial Expression of DmMextli_577-640

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640 (mxt Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-41-DmPABPC1_J

Plasmid

#146643

Purpose

Bacterial Expression of DmPABPC1

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1 (pAbp Fly)

Expression

Bacterial

Mutation

4 silent mutations compared to the sequence geven…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

Plasmid

#194725

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Mutation

D908A

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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