We narrowed to 6,025 results for: plasmid dna

1. prDNA spacer-LacOx50-20xMS2-Hyg

   Plasmid

   #210219

   Purpose

   Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Pert Heavy chain-SpyTag003

   Plasmid

   #216309

   Purpose

   Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. (365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)

   Plasmid

   #168188

   Purpose

   HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-nUbc6E-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Tras heavy chain-SpyTag003

   Plasmid

   #216293

   Purpose

   Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_SARS-CoV-Spike[WIV1 RBD]

   Plasmid

   #190087

   Purpose

   express chimeric SARS-CoV spike with the WIV-1 receptor binding domain

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   SARS-CoV S (S Synthetic)
   

   Use

   Tags

   Rhodopsin C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3_SARS-CoV-Spike[Khosta2 RBD]

   Plasmid

   #190083

   Purpose

   express chimeric SARS-CoV spike with the Khosta-2 receptor binding domain

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   SARS-CoV S (S Synthetic)
   

   Use

   Tags

   Rhodopsin C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3_SARS-CoV-Spike[Khosta1 RBD]

   Plasmid

   #190082

   Purpose

   express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   SARS-CoV S (S Synthetic)
   

   Use

   Tags

   Rhodopsin C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_SARS-CoV-Spike[BtKY72 RBD]

   Plasmid

   #190081

   Purpose

   express chimeric SARS-CoV spike with the BtKY72 receptor binding domain

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   SARS-CoV S (S Synthetic)
   

   Use

   Tags

   Rhodopsin C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3_SARS-CoV-Spike[PRD0038 RBD]

    Plasmid

    #190085

    Purpose

    express chimeric SARS-CoV spike with the PRD0038 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-hygro-eGFP-STOP-HEC1

    Plasmid

    #114026

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-STOP-HEC1 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Start codon of Hec1 is mutated to stop codon

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid

    #136451

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-HLA-Flag-natT1R3

    Plasmid

    #113950

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    residues 21-852

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-EGFP-AID-BubR1

    Plasmid

    #47330

    Purpose

    for plasmid integration using the Flp-In system. AID at N-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Use

    Tags

    AID and GFP

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Promoter

    Available since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT/TO-mNeonGreen-BRD4

    Plasmid

    #204692

    Purpose

    This plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HCMVgO-sfGFP (Merlin)

    Plasmid

    #136452

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid

    #136445

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-717). Codon-optimize…

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid

    #136453

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.2 mir-1-1 reporter mir16-1 wt

    Plasmid

    #46683

    Purpose

    Expresses wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1 wt (MIR16-1 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2 mir-1-1 reporter mir129-2-wt

    Plasmid

    #46730

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir129-2-wt (MIR129-2 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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