We narrowed to 6,456 results for: plasmid dna

1. pcDNA3-sPDGFRalpha-Fc-V242K

   Plasmid

   #136440

   Purpose

   Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   PDGFRalpha (PDGFRA Human)
   

   Use

   Tags

   Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

   Expression

   Mammalian

   Mutation

   Extracellular domain of PDGFR-alpha (a.a. 1-524) …

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-ER-EBFP2

   Plasmid

   #231183

   Purpose

   Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   ER-EBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5_miniTurbo-C12orf49

   Plasmid

   #155111

   Purpose

   transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   C12orf49 (SPRING1 Human)
   

   Use

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-ER-mRFP

   Plasmid

   #231176

   Purpose

   Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cells

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   ER-mRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5_C12orf49-miniTurbo

   Plasmid

   #155112

   Purpose

   transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   C12orf49 (SPRING1 Human)
   

   Use

   Tags

   3x Flag and miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

   Plasmid

   #69901

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. prDNA spacer-LacOx50-20xMS2-Hyg

   Plasmid

   #210219

   Purpose

   Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Pert Heavy chain-SpyTag003

   Plasmid

   #216309

   Purpose

   Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. (365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)

    Plasmid

    #168188

    Purpose

    HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nUbc6E-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Tras heavy chain-SpyTag003

    Plasmid

    #216293

    Purpose

    Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3_SARS-CoV-Spike[WIV1 RBD]

    Plasmid

    #190087

    Purpose

    express chimeric SARS-CoV spike with the WIV-1 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3_SARS-CoV-Spike[Khosta2 RBD]

    Plasmid

    #190083

    Purpose

    express chimeric SARS-CoV spike with the Khosta-2 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3_SARS-CoV-Spike[Khosta1 RBD]

    Plasmid

    #190082

    Purpose

    express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3_SARS-CoV-Spike[BtKY72 RBD]

    Plasmid

    #190081

    Purpose

    express chimeric SARS-CoV spike with the BtKY72 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3_SARS-CoV-Spike[PRD0038 RBD]

    Plasmid

    #190085

    Purpose

    express chimeric SARS-CoV spike with the PRD0038 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-hygro-eGFP-STOP-HEC1

    Plasmid

    #114026

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-STOP-HEC1 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Start codon of Hec1 is mutated to stop codon

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-HLA-Flag-natT1R3

    Plasmid

    #113950

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    residues 21-852

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-EGFP-AID-BubR1

    Plasmid

    #47330

    Purpose

    for plasmid integration using the Flp-In system. AID at N-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Use

    Tags

    AID and GFP

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Promoter

    Available since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid

    #136451

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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