We narrowed to 475 results for: chat

1. pdCas9-omega

   Plasmid

   #114632

   Purpose

   Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

   Depositor

   Anushree Chatterjee

   Article

   Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

   Insert

   dCas9-omega
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pANAP

   Plasmid

   #48696

   Purpose

   Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

   Depositor

   Peter Schultz

   Article

   Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

   Inserts

   8 copies of the amber suppressor tRNACUA-Leu
   AnapRS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant E.coli leucyl synthetase specific for Anap…

   Promoter

   CMV and human H1 promoter

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. deltaNp63alpha-FLAG

   Plasmid

   #26979

   Depositor

   David Sidransky

   Article

   Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

   Insert

   ΔNP63alpha (TP63 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   dominant negative isoform of p63; aa 1–587

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. iSpyMac

   Plasmid

   #155014

   Purpose

   Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

   Insert

   iSpyMac
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PID grafted onto SpyCas9 N-terminal domain, R221K…

   Promoter

   EF1ɑ

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIDTSmart-MbPylRS

   Plasmid

   #217361

   Purpose

   Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. barkeri pyrrolysyl-tRNA synthetase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET151-D-TOPO-PARP14-SΔK

   Plasmid

   #216658

   Purpose

   Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

   Depositor

   Herwig Schüler

   Article

   Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

   Insert

   PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   Deletion starting from Q1390 to D1448 and substit…

   Promoter

   T7

   Available since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-p27(kip1)

   Plasmid

   #50026

   Purpose

   pENTR vector for human cyclin-dependent kinase inhibitor 1B

   Depositor

   Jamie Davies

   Article

   Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

   Insert

   p27(kip1) (CDKN1B Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pIDTsmart-RC2-delVP2-VP2-454TAG

   Plasmid

   #240060

   Purpose

   Express AAV2 capsid with ncAA mutant at site 454 of VP2

   Depositor

   Abhishek Chatterjee

   Article

   Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

   Inserts

   RC2-DelVP2
   VP2-454-TAG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIDTsmart-RC2-delVP1-VP1-454TAG

   Plasmid

   #240059

   Purpose

   Express AAV2 capsid with ncAA mutant at site 454 of VP1

   Depositor

   Abhishek Chatterjee

   Article

   Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

   Inserts

   RC2-DelVP1
   VP1-454-TAG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PC2328

    Plasmid

    #232089

    Purpose

    Mammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.

    Depositor

    Pranam Chatterjee

    Article

    Hong et al Nat Commun. 2025 Apr 15;16(1):3555. doi:

    Insert

    duAb with cloning Esp3I restriction site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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