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Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRTagsExpressionBacterialMutationPromoterTet promoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
deltaNp63alpha-FLAG
Plasmid#26979DepositorInsertΔNP63alpha (TP63 Human)
UseTagsFLAGExpressionMammalianMutationdominant negative isoform of p63; aa 1–587PromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
iSpyMac
Plasmid#155014PurposeExpresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cellsDepositorInsertiSpyMac
UseTagsExpressionMammalianMutationPID grafted onto SpyCas9 N-terminal domain, R221K…PromoterEF1ɑAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIDTSmart-MbPylRS
Plasmid#217361PurposeExpresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmidDepositorInsertM. barkeri pyrrolysyl-tRNA synthetase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-D-TOPO-PARP14-SΔK
Plasmid#216658PurposeExpresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.DepositorInsertPARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
UseTags6xHis-V5-TEVExpressionBacterialMutationDeletion starting from Q1390 to D1448 and substit…PromoterT7Available sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p27(kip1)
Plasmid#50026PurposepENTR vector for human cyclin-dependent kinase inhibitor 1BDepositorInsertp27(kip1) (CDKN1B Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-delVP2-VP2-454TAG
Plasmid#240060PurposeExpress AAV2 capsid with ncAA mutant at site 454 of VP2DepositorInsertsRC2-DelVP2
VP2-454-TAG
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIDTsmart-RC2-delVP1-VP1-454TAG
Plasmid#240059PurposeExpress AAV2 capsid with ncAA mutant at site 454 of VP1DepositorInsertsRC2-DelVP1
VP1-454-TAG
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
PC2328
Plasmid#232089PurposeMammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.DepositorInsertduAb with cloning Esp3I restriction site
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only