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Plasmid#185333PurposeBacterial expression of AUP1 with GST fusion protein for binding assaysDepositorInsertAUP1 (AUP1 Human)
UseTagsGSTExpressionBacterialMutationaa 292-410 only, with mutation A379DPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
UBE2G2 shRNA
Plasmid#201813PurposesiRNADepositorInsertUBE2G2 (UBE2G2 Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCINeo CD3 delta
Plasmid#201814PurposeMammalian ExpressionDepositorInsertCD3-delta (Cd3d Mouse)
UseTagsHAExpressionMammalianMutationnonePromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-Cys-HA-Ubiquitin
Plasmid#185356PurposeIn vitro ubiquitination assaysDepositorInsertUbiquitin
UseTagsGST, HA(x2)ExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-G2BRSCR
Plasmid#185345PurposeExpression of soluble G2BR with scrambled sequenceDepositorInsertscrambled gp78 G2BR sequence
UseTagsEGFPExpressionMammalianMutationscrambled sequencePromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-AUP1 A397D
Plasmid#185342PurposeAssessing G2BR function in AUP1DepositorInsertAUP1 (AUP1 Human)
UseTagsFlag, 6xHisExpressionMammalianMutationA397DPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2
Plasmid#185346PurposeMembrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2DepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG
Plasmid#185347PurposeMembrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tagDepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-G2BRgp78
Plasmid#185344PurposeExpression of soluble G2BR, amino acids 575-600 of human gp78DepositorInsertgp78 aa 574-600 (G2BR) (AMFR Human)
UseTagsEGFPExpressionMammalianMutationaa 575-600 onlyPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-G2BRAUP1
Plasmid#185343PurposeExpression of soluble G2BR, amino acids 378-404 of human AUP1DepositorInsertAUP1 aa 378-404 (G2BR) (AUP1 Human)
UseTagsEGFPExpressionMammalianMutationaa 378-404 onlyPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST-AUP1 379-410:Ube2G2
Plasmid#185335PurposeUsed for co-expression of the AUP1 G2BR with UBE2G2 for crystallographyDepositorInsertAUP1 aa 379-410, Ube2G2 (AUP1 Human, Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE Y165A
Plasmid#139428PurposeGST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationChanged tyrosine 165 to an alaninePromoterT7 promoter-1Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE P199A
Plasmid#139427PurposeGST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationChanged proline 199 to an alaninePromoterT7 promoter-1Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE I137A
Plasmid#139319PurposeGST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationChanged isoleucine 137 to an alaninePromoterT7 promoter-1Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1 AO7R
Plasmid#137842PurposeGST tagged AO7 encoding aa 126-216 for expression in bacteriaDepositorInsertAO7 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1509
Plasmid#62363PurposepFA6a BirA-heh2 linker-SSH1::HIS5DepositorInsertBirA-heh2-Ssh1
UseTagsExpressionYeastMutationPromoterGal1Available sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1668
Plasmid#112042PurposeCEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URADepositorInsertFET3
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1669
Plasmid#112043PurposeCEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URADepositorInsertNSG2
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1672
Plasmid#112046PurposeCEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URADepositorInsertPST1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1674
Plasmid#112048PurposeCEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URADepositorInsertSWP1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pSB700 Cerulean-Weissman scaffold
Plasmid#79377PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertscaffold
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP
Plasmid#188899PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR101
Plasmid#187241PurposeLentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector for Perturb-seq with mU6 promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro
Plasmid#188768PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR104
Plasmid#187243PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR98
Plasmid#187239PurposeCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sitesDepositorInsertCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-BFP
Plasmid#188775PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry
Plasmid#188779PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsZim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP
Plasmid#188777PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP
Plasmid#188778PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP
Plasmid#46910PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFPDepositorInsertdCas9-BFP fusion
UseCRISPR and LentiviralTags2xNLS, BFP, and HAExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMLS-SV40-EGFP
Plasmid#46919PurposeTarget EGFP gene that is stably integrated into HEK293 genomeDepositorInsertGFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJR103
Plasmid#187242PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP
Plasmid#188902PurposedCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertdCas9-KRAB(Kox1)-MeCP2
UseLentiviralTagsNLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…ExpressionMutationPromoterSFFVAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-EGFP
Plasmid#188900PurposeHuman lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-BFP
Plasmid#188776PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCXCR4-2
Plasmid#46917PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 geneDepositorInsertsUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only