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pCDNA3-FLAG-Regnase-3-HA_full-length

Plasmid

#222656

Purpose

Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 WT

Plasmid

#222651

Purpose

For in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pOG44_FLP_recombinase (pEHA1338)

Plasmid

#209087

Purpose

FLP recombinase for FRT-mediated integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Insert

FLP recombinase

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-Zc3h12a 3’UTR

Plasmid

#222662

Purpose

Luciferase reporter vector containing mouse Zc3h12a 3'UTR

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a 3'UTR (Zc3h12a Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-547 aa

Plasmid

#222657

Purpose

Mammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-547 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated histidine 548 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-409 aa

Plasmid

#222658

Purpose

Mammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-409 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated proline 410 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-244 aa

Plasmid

#222659

Purpose

Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-244 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated asparagine 245 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-Regnase-3 WT-T2A-copGFP

Plasmid

#222660

Purpose

Lentiviral vector to express mouse Regnase-3 WT

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

mutated stop codon prior to a T2A peptide

Promoter

EF1α

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-1 D141N

Plasmid

#222654

Purpose

Mammalian expression vector to express mouse Regnase-1 D141N mutant

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 D252N

Plasmid

#222652

Purpose

For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT

Plasmid

#222649

Purpose

For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pScalps-Puro-FLAG_HA-Regnase-1 D141N

Plasmid

#222663

Purpose

Lentiviral vector to express mouse Regnase-1 D141N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

SFFV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pScalps-Puro-FLAG-HA-Regnase-3 D252N

Plasmid

#222664

Purpose

Lentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

SFFV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-Regnase-3 D252N-T2A-copGFP

Plasmid

#222661

Purpose

Lentiviral vector to express mouse Regnase-3 D252N

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

EF1α

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N

Plasmid

#222650

Purpose

For in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2886

Plasmid

#143655

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GLI3 (GLI3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

Plasmid

#209077

Purpose

Ngn2 mediated cortical neuron induction, dox induction is included

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
SNAPtag

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

Plasmid

#209084

Purpose

Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
BirA

Expression

Mammalian

Mutation

human codon optimized

Promoter

hSyn

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

Plasmid

#209083

Purpose

SNCA (Avi tagged) expression along with BirA ligase

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
BirA

Tags

Avi

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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