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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 188 results for: pef gfp

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Showing: 101 - 120 of 188 results

  1. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176459
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Publication
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176459-plasmid-map-sequence-id-346304

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  2. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176460
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Publication
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176460-plasmid-map-sequence-id-346305

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  6. pEF-XMAS-2xStop

    Plasmid
    #164412
    Purpose
    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.
    Depositor
    Xiao Wang
    Publication
    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    164412-plasmid-map-sequence-id-338860

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  8. pEF1α-ancestral-retroCHMP3-HA

    Plasmid
    #154248
    Purpose
    expresses ancestral New World monkey retroCHMP3 in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    reconstructed ancestral sequence of New World monkey retroCHMP3
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    154248-plasmid-map-sequence-id-328994

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  13. pCFJ1320

    Plasmid
    #84829
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84829-plasmid-map-sequence-id-163669

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  18. pEF1α-owl-monkey-retroCHMP3(148)-HA

    Plasmid
    #154252
    Purpose
    expresses prematurely truncated owl monkey retroCHMP3 in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    retroCHMP3
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C-terminal truncation of retroCHMP3 at amino acid…
    Promoter
    EF1-alpha
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    154252-plasmid-map-sequence-id-333885

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  19. pEF1α-squirrel-monkey-CHMP3(155)-HA

    Plasmid
    #154173
    Purpose
    expresses squirrel monkey CHMP3(155) in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHMP3(155)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C-terminal truncation of CHMP3 at amino acid 155
    Promoter
    EF1-alpha
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154173-plasmid-map-sequence-id-308881

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  20. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

    Plasmid
    #100944
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Synthetic, Human)
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    F636A, E660A, E725A, R295A
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100944-plasmid-map-sequence-id-206112

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Showing: 101 - 120 of 188 results