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p2CT-His-MBP-Rca_Cas13a_WT

Plasmid

#91870

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

RcaCas13a (RcaC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA AAVS1

Plasmid

#128119

Purpose

Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA AAVS1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pXS-dTomato

Plasmid

#117387

Purpose

pGEN-mcs with a modular dTomato expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

dTomato

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate1-N1

Plasmid

#31870

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate2-N1

Plasmid

#31871

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (insertion 5)

Plasmid

#128116

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (insertion 5)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (N39A)

Plasmid

#128117

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (N39A)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (wtAcrIIA4)

Plasmid

#128115

Purpose

Expresses SpyCas9 fused to wild-type AcrIIA4

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (wtAcrIIA4)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA EMX1

Plasmid

#128121

Purpose

Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA EMX1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSuperRetro mouse Hic-5

Plasmid

#11187

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 shRNA (Tgfb1i1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1E04

Plasmid

#71076

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sirt4 (Sirt4 Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF137

Plasmid

#13187

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF137

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap1

Plasmid

#231879

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap1

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap1

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3

Plasmid

#231874

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, D133R, K143R

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 P+

Plasmid

#231872

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 P+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 P+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S122P, T131F, A141P, E143D

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3 W

Plasmid

#231876

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3 W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3 W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, N126W, D133R, K143R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R2

Plasmid

#231870

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

K118R, K140R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-GeneTRAP

Plasmid

#218187

Purpose

To KO and genetrap mouse Vamp2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS18

Plasmid

#218718

Purpose

Expresses N-terminally EGFP-tagged BBS18 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS18

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-MARK4(v3)

Plasmid

#166187

Purpose

Gateway entry vector for N. furzeri MARK4

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

MARK4(v3)

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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