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pHAGE DN Cullin3

Plasmid

#41913

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

Cullin 3 (CUL3 Human)

Use

Lentiviral

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-jGCaMP7b-XN

Plasmid

#178362

Purpose

Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.

Depositor

Xiaodong Liu

Article

Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XN

Expression

Mammalian

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-indRecA56

Plasmid

#102294

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Use

CRISPR

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-K298M-Y419F-Y530F

Plasmid

#140321

Purpose

Expresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

K298M-Y419F-Y530F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin4B

Plasmid

#41915

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL4B (CUL4B Human)

Use

Lentiviral

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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PH-Btk(R28C)-GFP

Plasmid

#51464

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

BTK (aa 1–177) (BTK Human)

Tags

GFP

Expression

Mammalian

Mutation

R28C

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pPong_v2

Plasmid

#47101

Purpose

Expresses the Pong ORF1 and transposase from rice to allow mPing movement. Contains ORF1 and transposase driven by a native soybean promoter. Contains mPing and BAR for basta selection.

Depositor

Wayne Parrott

Article

Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

Inserts

mPing
Pong Open Reading Frame 1 (ORF1)
Pong Transposase

Use

Plant expression

Promoter

GmUbi_AP, GmUbi_XS, and no promoter

Available Since

Oct. 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pRevTRE MKL1-N100

Plasmid

#19848

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1-N100 (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Mutation

deleted amino acids 1-100

Available Since

Jan. 13, 2009

Availability

Academic Institutions and Nonprofits only
eGFP-HA_BASU

Plasmid

#153996

Purpose

Express eGFP gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

E-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S32-KD

Plasmid

#140311

Purpose

Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Contains only the SH3, SH2, and KD regions

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-KD

Plasmid

#140297

Purpose

Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCRE-iRFP670

Plasmid

#82696

Purpose

Expresses CRE and iRFP670 under two separate PGK promoters.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Insert

CRE, iRFP670

Use

Cre/Lox

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3Basic-miR126-EGFL7-Promoter

Plasmid

#32244

Depositor

Charles Lowenstein

Article

Harris et al Arterioscler Thromb Vasc Biol. 2010 Oct;

Insert

miR-126 Promoter 5' UTR (MIR126, EGFL7 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Promoter region 5' UTR

Promoter

5' UTR

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pgRGFP

Plasmid

#82695

Purpose

Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

PGK, U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4ΔCTD

Plasmid

#157797

Purpose

Expression of human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4ΔCTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-FAM86A

Plasmid

#85117

Purpose

bacterial expression of FAM86A

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EEF2KMT (FAM86A Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 S454A

Plasmid

#19844

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S454A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 454 to Alanine

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pDEST40-2XHA-Src-K298M-Y419F-Y530F

Plasmid

#140320

Purpose

Expresses HA-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

K298M-Y419F-Y530F

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-KD

Plasmid

#140312

Purpose

Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Contains only the KD region

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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