We narrowed to 4,125 results for: 272

1. DRD5-Tango

   Plasmid

   #66272

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   DRD5 (DRD5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dom neg CstF64

   Plasmid

   #127250

   Purpose

   Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   CstF64 delta 282 (CSTF2 Human)
   

   Use

   Other

   Tags

   Expression

   Mammalian

   Mutation

   insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-Nkx2-1*/Neo

   Plasmid

   #31272

   Depositor

   Monte Winslow, Tyler Jacks

   Article

   Winslow et al Nature. 2011 May 5. 473(7345):101-4.

   Insert

   Nkx2-1* (Nkx2-1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A shNkx2-1 insensitive Nkx2-1 cDNA was created by…

   Promoter

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-hs-NRAS

   Plasmid

   #52728

   Purpose

   retroviral expression of human NRAS

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   NRAS (NRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMXs-hs-3xHA-NHL only-LIN-41

   Plasmid

   #52723

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing only the NHL repeats and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   NHL-only contains only an initiating methionine a…

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-hs-3xHA-7CtoA-LIN-41

   Plasmid

   #52726

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   changed cysteines 12, 15, 61, 66, 69, 91, and 94 …

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2T-U6-sgCTG

   Plasmid

   #232723

   Purpose

   SpCas9 guide RNA targeting CAG repeats for C-to-T base editing

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   SpCas9 gRNA targeting CAG repeats
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-FKBP-IRSp53(trunc N1)

   Plasmid

   #221272

   Purpose

   Expression of IRSp53 N-terminal truncation 1 (257-375) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   IRSp53 N-terminal truncation 1 (257-375) (Baiap2 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO_HSV.NWS.Ollas_mCherry-NLS

   Plasmid

   #178272

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.NWS.Ollas and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. frameshift ELL2

    Plasmid

    #127257

    Purpose

    For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    XhoI site between the 1st &2nd Met was filled…

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. II-ATCC

    Plasmid

    #43272

    Depositor

    Chad Cowan, Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Insert

    II-ATCC
    

    Use

    Bacterial cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pDONR221-TMEM165

    Plasmid

    #132272

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    TMEM165 (TMEM165 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLKO.1 puro CXCR4 siRNA-2

    Plasmid

    #12272

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    CXCR4 siRNA (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPR182-DuET

    Plasmid

    #213272

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR182 (GPR182 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T-CMV-ABE8e_dNRCH-BlastR

    Plasmid

    #232720

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    ABE8e-dCas9(NRCH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    within Cas9 D10A H840A

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1.11.1

    Plasmid

    #223272

    Purpose

    Encodes SARS-CoV-2 spike JN.1.11.1 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike JN.1.11.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223 SARS-CoV-2 ORF3B

    Plasmid

    #141272

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3B
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. WT USP7 TRAF

    Plasmid

    #227226

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 62-205

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHLSec2-irisin-his

    Plasmid

    #122729

    Purpose

    Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Mouse, Bovine, Human, Rat)
    

    Use

    Tags

    his and signal sequence for secretion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PLL5.0-Anillin ShRNA-GFP-AnillinFL

    Plasmid

    #187272

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6, CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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