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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,772 results for: PGK

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Showing: 1181 - 1200 of 2772 results

  7. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #84257
    Purpose
    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    84257-plasmid-map-sequence-id-160891

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  8. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

    Plasmid
    #191391
    Purpose
    Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hCdt1(30-120)
    Use
    CRISPR and Synthetic Biology
    Tags
    mScarlet-I
    Expression
    Mammalian
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191391-plasmid-map-sequence-id-378101

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  15. ATP1A1_T804N_hPGK1_EBFP_Donor

    Plasmid
    #187455
    Purpose
    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1-T804N EBFP donor (ATP1A1 Human)
    Expression
    Mammalian
    Promoter
    hPGK1
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187455-plasmid-map-sequence-id-367518

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  17. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

    Plasmid
    #191389
    Purpose
    Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hCdt1(1-100)
    Tags
    mScarlet-I
    Expression
    Mammalian
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191389-plasmid-map-sequence-id-378099

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  18. pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

    Plasmid
    #156431
    Purpose
    Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Elphege Nora
    Publication
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAD21, Halotag (Rad21 Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    156431-plasmid-map-sequence-id-310238

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Showing: 1181 - 1200 of 2772 results