We narrowed to 18,398 results for: Mut

1. pRRL-VinculinTS-I997A

   Plasmid

   #111832

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A) (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changed vinculin isoleucine 997 to alanine

   Promoter

   CMV

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_LifeAct-iBlueberry

   Plasmid

   #73176

   Purpose

   Expressing iBlueberry and mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

   Insert

   LifeAct-iBlueberry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutation E177Q in iBlueberry

   Promoter

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-L3-SpCas9-HF1-2A-EGFP-L2

   Plasmid

   #141048

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF1-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCK01_PalkS_alkS

   Plasmid

   #166502

   Purpose

   Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

   Depositor

   Sven Panke

   Article

   Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

   Insert

   alkS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   L401G

   Promoter

   PalkS

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

   Plasmid

   #182009

   Purpose

   CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   IgKchL_HA_STf_myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJH776_pJH_LldR_PlldR-sfGFP.3opal

   Plasmid

   #163735

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding three opal codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.3TGA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp with first three leucine codons mutated to am…

   Promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEM705 Gst (Ser)

   Plasmid

   #109354

   Purpose

   GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gst chimera
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine at position 391 to Serine (Pertu…

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

   Plasmid

   #135454

   Purpose

   Bacterial expression of TEVΔ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
   

   Use

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability. Truncated form…

   Promoter

   tac promoter

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE_tau-LacZ::tTA-H100Y

   Plasmid

   #34927

   Depositor

   Mark Mayford

   Article

   Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

   Inserts

   tau (MAPT Bovine)
   tTA
   

   Use

   Mouse Targeting

   Tags

   LacZ

   Expression

   Mammalian

   Mutation

   Made doxycycline insensitive by introduction of a…

   Promoter

   tetO

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. YF-Rac1(CA)

    Plasmid

    #20150

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Rac1(CA) (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    constitutively active mutation

    Promoter

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50456

    Purpose

    Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Insert

    rM3D-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. (R42A) Cyclin B1-GFP (62)

    Plasmid

    #61849

    Purpose

    mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

    Depositor

    Jonathon Pines

    Article

    Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

    Insert

    cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    V5A R42A

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5 HA3-eDHFR-T2A-puro

    Plasmid

    #86395

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A puro
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A puro

    Expression

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Promoter

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX601‐MHP1‐miniABEmaxNG‐N2‐(GG)

    Plasmid

    #187067

    Purpose

    Gp41-1 Split N-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    miniABEmaxNG-Gp41
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MHP1

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX601‐MHP1‐ABEmaxC2‐NG‐E53 ogRNA

    Plasmid

    #187068

    Purpose

    Gp41-1 Split C-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    Gp41-ABEmaxC2‐NG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MHP1

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEB2-DsRed-Express2

    Plasmid

    #103999

    Purpose

    Plasmid encoding DsRed-Express2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    DsRed-Express2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET19b+H2B(T116C)

    Plasmid

    #157808

    Purpose

    Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H2B(T116C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T116C

    Promoter

    T7

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA.PKD.K/W

    Plasmid

    #10809

    Depositor

    Alex Toker

    Article

    Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

    Insert

    PKD (PRKD1 Human)
    

    Use

    Tags

    HA and Myc (inserted between amino acids 33&3…

    Expression

    Mammalian

    Mutation

    Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

    Promoter

    CMV

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pME-PLIN2-tdTOMATO

    Plasmid

    #172533

    Purpose

    PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F, and R415H mutations in …

    Promoter

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAID 1.1 N-T2A-Bsr

    Plasmid

    #105985

    Purpose

    To make an auxin inducible degron mutant

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

    Insert

    T2A-Bsr
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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