We narrowed to 3,154 results for: BFP

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pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

Plasmid

#193968

Purpose

Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

hPol eta (POLH Human)

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-dead Tet1CD-P2A-BFP

Plasmid

#108246

Purpose

Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-dead Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

Promoter

Ubc

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-BLM-UGI-T2A-mTagBFP2

Plasmid

#221222

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-BLM-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCW-Tet-Cas9-BFP-PGK-Blast

Plasmid

#196720

Purpose

Tet-inducible expression of Cas9-T2A-TagBFP

Depositor

Bernhard Schmierer

Insert

Cas9-T2A-TagBFP

Use

CRISPR and Lentiviral

Tags

3xFLAG-SV40NLS and Nucleoplasmin NLS

Promoter

TRE, PGK

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

Plasmid

#102244

Purpose

Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

Depositor

Jacob Corn

Insert

dCas9-HA-BFP-KRAB-NLS

Use

CRISPR and Lentiviral

Tags

HA-TagBFP-KRAB-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

Plasmid

#171588

Purpose

Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

Bxb1 integrase
iCasp9
BFP
rtTA3G

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

Plasmid

#236231

Purpose

AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX1-TET-VP16-ZF43-2A-tagBFP-bGH_DIVrtTA_pAMB282

Plasmid

#246379

Purpose

ZFa Lentivirus expressing VP16-ZF43-2A-TagBFP from the TET promoter with rtTA expressed divergently

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP

Plasmid

#232608

Purpose

Pan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

GUR-3 (gur-3 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

Plasmid

#221225

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

Plasmid

#205160

Purpose

express β-ACTIN-3′UTR-8xMS2

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

β-ACTIN-3′UTR-24xMS2

Tags

tagBFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP

Plasmid

#64323

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-EBFP2

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

Plasmid

#226009

Purpose

DjCas13d guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

Plasmid

#64216

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

Plasmid

#178707

Purpose

AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

Dreadd-hM3Dq-HA-P2A-tBFP

Use

AAV

Promoter

hSyn

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

Plasmid

#173029

Purpose

For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

Depositor

Mohamed Fareh

Article

Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

Insert

pspCas13b

Use

CRISPR and Lentiviral

Tags

3xFLAG, BFP, HIV NES, and T2A

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-BFP

Plasmid

#188775

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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