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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,401 results for: D10

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Showing: 1201 - 1220 of 2401 results

  5. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68417-plasmid-map-sequence-id-120195

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  13. AICSDP-19:LAMP1-mEGFP

    Plasmid
    #101782
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1
    Depositor
    Allen Institute for Cell Science
    Insert
    LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Available Since
    Nov. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101782-plasmid-map-sequence-id-198613

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  15. pH-A3A-PBE

    Plasmid
    #119774
    Purpose
    Conversion of C to T in rice plants transformation
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-NLS-UGI-NLS
    Tags
    NLS (flanking UGI)
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A in wild type SpCas9
    Promoter
    maize Ubiquitin-1
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119774-plasmid-map-sequence-id-257661

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Showing: 1201 - 1220 of 2401 results