We narrowed to 2,288 results for: D10

1. pJMP8930

   Plasmid

   #220905

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p35S-A3A-PBE

   Plasmid

   #119773

   Purpose

   Conversion of C to T in potato

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS-UGI-NLS
   

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   CaMv 2x35S

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV2-CAGIG-LAMP1-mGL

   Plasmid

   #222394

   Purpose

   To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.

   Depositor

   Pantelis Tsoulfas

   Insert

   LAMP1 and mGreenlantern (LAMP1 Homo Sapiens, Human, Synthetic)
   

   Use

   AAV

   Tags

   LAMP1 and mGreenLantern

   Expression

   Mammalian

   Promoter

   CAG or CAGIG

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFLAG-dCas9/pTEF1p-CYC1t

   Plasmid

   #62190

   Purpose

   Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   human codon-optimized, D10A, H840A

   Promoter

   TEF1 promoter

   Available Since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. A3A-Gam

   Plasmid

   #119769

   Purpose

   Conversion of C to T monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
   

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   maize Ubiquitin-1

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_m4_Blasto

   Plasmid

   #167864

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. dABE

   Plasmid

   #124447

   Purpose

   Expresses dead Cas9 (dCas9)-ABE7.10

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   dABE7.10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKin1A

   Plasmid

   #31607

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   kinesin-1A (Kif5a Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAL2430

   Plasmid

   #36829

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HDAC2-TALEN_Left (Hdac2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pnxABE-flgn

    Plasmid

    #187932

    Purpose

    A-to-G base editor

    Depositor

    Yi Xiao

    Article

    et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

    Insert

    ecTadA(8e)-nxCas9 (D10A)
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p35S-PBE

    Plasmid

    #119772

    Purpose

    Conversion of C to T in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    NLS-APOBEC1-nCas9-UGI-NLS
    

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    CaMv 2x35S

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TAL2431

    Plasmid

    #36830

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HDAC2-TALEN_Right (Hdac2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. NAX_CAGGS_CAS9_m4_Puro

    Plasmid

    #167871

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SlugBE4max

    Plasmid

    #163799

    Purpose

    Expresses AncBE4max and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. JEH127

    Plasmid

    #179299

    Purpose

    Expresses dCas9 fused to VPR

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-VPR
    

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUHD Cdk2 WT HA

    Plasmid

    #27650

    Depositor

    Greg Enders

    Article

    Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

    Insert

    Cdk2 (CDK2 Human)
    

    Tags

    HA (YDVPDYA)

    Expression

    Mammalian

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCXLE-dCas9GFP-shP53

    Plasmid

    #102906

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9GFP-shP53
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQTEV-STX10

    Plasmid

    #31572

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    syntaxin 10 (STX10 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4095420

    Plasmid

    #149523

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    d1-745 d1063-1945

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pRS315e_pGal-dCas9-SH3

    Plasmid

    #79613

    Purpose

    Expresses dCas9-SH3 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Tags

    SH3 domain

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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