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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,488 results for: ESP

Showing: 1201 - 1220 of 10488 results

  2. psiCHECK2-let-7 MT

    Plasmid
    #78261
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated let-7 target site
    Use
    Luciferase; Microrna activity
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CTCC to GA…
    Available Since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78261-plasmid-map-sequence-id-149478

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  3. pMSCV-BLU-VIPR

    Plasmid
    #220497
    Purpose
    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220497-plasmid-map-sequence-id-442817

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  5. pGBW-m4046785

    Plasmid
    #145684
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145684-plasmid-map-sequence-id-297841

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  9. pHYP-NPC-10H

    Plasmid
    #162789
    Purpose
    SARS-CoV-2 Nucleocapsid Protein expression in E.coli
    Depositor
    Ivan Vorobiev
    Article
    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771
    Insert
    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    10xHis
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    162789-plasmid-map-sequence-id-333793

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  10. psiCHECK2-miR-34 MT

    Plasmid
    #78259
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated miR-34 target site
    Use
    Luciferase; Microrna activity
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CCGT to GG…
    Available Since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78259-plasmid-map-sequence-id-149444

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  11. pQE80l B-CN-B

    Plasmid
    #196477
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN
    Depositor
    Fei Sun
    Article
    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1
    Insert
    B-CN-B
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196477-plasmid-map-sequence-id-387241

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  12. pGBW-m4046810

    Plasmid
    #149251
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149251-plasmid-map-sequence-id-357431

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  16. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

    Plasmid
    #162949
    Purpose
    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
    Use
    Synthetic Biology
    Available Since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162949-plasmid-map-sequence-id-330549

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  18. pSTAP-seq_fly-zfh1

    Plasmid
    #86381
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer
    Use
    Stap-seq screening vector
    Available Since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86381-plasmid-map-sequence-id-173935

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  19. pSTAP-seq_fly-tj

    Plasmid
    #86386
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster tj enhancer
    Use
    Stap-seq screening vector
    Available Since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86386-plasmid-map-sequence-id-173933

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Showing: 1201 - 1220 of 10488 results