We narrowed to 2,528 results for: PTS

1. pinducer 20 DN-KASH

   Plasmid

   #125554

   Purpose

   Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Lentiviral

   Tags

   mcherry

   Expression

   Mutation

   Truncated form: only the KASH domain of nesprin-…

   Promoter

   tetracycline responsive element

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. anxA2-GFP

   Plasmid

   #107196

   Purpose

   Expresses human annexin A2-GFP fusion protein for live cell imaging

   Depositor

   Ursula Rescher, Volker Gerke

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A2 (ANXA2 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Ala residue 65 in human sequence replaced by Glu …

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD40-His/V5-c-MycT58A

   Plasmid

   #45598

   Depositor

   Rosalie Sears

   Article

   Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

   Insert

   c-Myc T58A (Myc Mouse)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   T58A

   Promoter

   CMV

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

4. DENV4 Rluc Reporter GND mutant Replicon

   Plasmid

   #118586

   Purpose

   For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

   Depositor

   Radhakrishnan Padmanabhan

   Article

   Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

   Insert

   DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
   

   Use

   Yeast-e. col shuttle vector prs424

   Tags

   Expression

   Mutation

   The conserved GDD in the NS5 polymerase gene muta…

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR FL CPAP RNAi resistant

   Plasmid

   #46390

   Depositor

   Pierre Gonczy

   Article

   Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

   Insert

   CPAP RNAi resistant mutant (CENPJ Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   silent mutation that renders it resistant to siRN…

   Promoter

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pD40-His/V5-c-Myc

   Plasmid

   #45597

   Depositor

   Rosalie Sears

   Article

   Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

   Insert

   c-Myc (Myc Mouse)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-OSF-PP-human-CHMP3(150)

   Plasmid

   #154181

   Purpose

   expresses human CHMP3(150) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3 (CHMP3 Human)
   

   Use

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   C-terminal truncation of CHMP3 at amino acid 150

   Promoter

   CAG

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1-PHD2

   Plasmid

   #21401

   Depositor

   Eric Metzen

   Article

   Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pD40-His/V5-c-MycS62A

   Plasmid

   #45599

   Depositor

   Rosalie Sears

   Article

   Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

   Insert

   c-Myc S62A (Myc Mouse)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   S62A

   Promoter

   CMV

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

10. WT MetNIQ in pBAD

    Plasmid

    #118253

    Purpose

    Wild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala)

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. N229A (EcQ) MetNIQ in pBAD

    Plasmid

    #118257

    Purpose

    N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (N229A) (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 229 to Alanine (Ala) and…

    Promoter

    None and araBAD Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

    Plasmid

    #118581

    Purpose

    N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 229 to Alanine (Ala) and…

    Promoter

    None and araBAD Promotoer

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. N295A (EcN) MetNIQ in pBAD

    Plasmid

    #118256

    Purpose

    N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 295 to Alanine (Ala)

    Promoter

    None and araBAD Promotoer

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid

    #118260

    Purpose

    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Use

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV(WT)-mouse-full-length-CHMP3-HA

    Plasmid

    #154175

    Purpose

    expresses mouse CHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (Chmp3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1α-mouse-CHMP3(147)-HA

    Plasmid

    #154177

    Purpose

    expresses mouse CHMP3(147) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (Chmp3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 147

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOTTC293 - pAAV EF1a V5-synuclein (WT)

    Plasmid

    #60057

    Purpose

    An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    alpha-synuclein (SNCA Human, human)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1 Full length Importin beta

    Plasmid

    #106941

    Purpose

    Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

    Insert

    human Importin beta-1 (KPNB1) (KPNB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-GAL4-UAS-huCD25- IRES-mCherry-PGK-BFP

    Plasmid

    #223211

    Purpose

    Lentiviral vector - mCherry reporter and huCD25 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    CD25 (IL2RA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFPC1-hVAP-A

    Plasmid

    #104447

    Purpose

    Expression of human VAP-A fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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