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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Article

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35

Plasmid

#87342

Purpose

To express human Nup35 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

nucleoporin 35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEERM1 Km

Plasmid

#64024

Purpose

Plasmid for integration into psbA2 site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PpsbA2

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_3xFLAG_V5_ mmIfih1_V5

Plasmid

#160110

Purpose

Expresses murine Ifih1 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Ifih1 (Ifih1 Mouse)

Use

Lentiviral

Tags

3xFLAG, V5 and V5

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8.17-m

Plasmid

#136298

Purpose

expresses ABE8.17-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.17-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pHPdesteGFP

Plasmid

#24566

Depositor

Ingrid Lohmann

Article

Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

Insert

Use

Reporter construct

Tags

eGFP

Available Since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-StrepHA-SUMO2

Plasmid

#66867

Purpose

Mammalian expression of SUMO2 with a strep and an HA tag

Depositor

Ying Liu

Article

Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

Insert

SUMO2 (SUMO2 Human)

Tags

HA and Strep

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-HxB.L

Plasmid

#65414

Purpose

contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

Depositor

Harold Erickson

Article

Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

Insert

TNC large splice variant (TNC Human)

Expression

Bacterial

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-BZLF1

Plasmid

#72637

Purpose

Mammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulator

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BZLF1

Expression

Mammalian

Mutation

Q163R

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pTag-RFP-C-h-Rab11a

Plasmid

#79806

Purpose

Express TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Tags

TagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pRDV5 containing Sb-loop

Plasmid

#132695

Purpose

pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES2-mCherry-p53 deltaN

Plasmid

#49243

Purpose

expresses human p53 deltaN and mCherry in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 deltaN (TP53 Human)

Tags

IRES-mCherry

Expression

Mammalian

Mutation

deltaN ( lacks 39 residues at the N-terminus)

Promoter

CMV

Available Since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pTOX8

Plasmid

#127453

Purpose

Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
tsPurple chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

apFAB46 and pRha

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pM-Cas12a

Plasmid

#196291

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)

Use

CRISPR

Tags

Flag

Expression

Plant

Mutation

See depositor comments below

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1

Plasmid

#175049

Purpose

Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pc DNA FRT TO- APP695

Plasmid

#114193

Purpose

Creation of a stable cell line expressing APP mutation (AD in vitro study)

Depositor

Aleksandra Radenovic

Insert

Amyloid-Precursor-Protein (APP Human)

Expression

Mammalian

Mutation

wild type splice variant 695: 695AA (2085bp, lack…

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-FNIP2

Plasmid

#72295

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3 - hSRSF6

Plasmid

#120419

Purpose

overexpression of human SRSF6

Depositor

Gillian Bates, Andreas Neueder

Article

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (SRSF6 Human)

Expression

Mammalian

Promoter

CMV immediate/early

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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PTEN shRNA

Plasmid

#86645

Purpose

expression of shRNA targeting PTEN

Depositor

Jaime Modiano

Article

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (PTEN Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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