We narrowed to 1,335 results for: piggyBac

1. pB-CAGGS-dCas9

   Plasmid

   #110823

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   George Church, Alejandro Chavez

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB-mCherry-DMDEx23-eGFP

   Plasmid

   #211366

   Purpose

   Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

   Depositor

   Ulrich Lächelt

   Article

   Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

   Insert

   mCherry-DMDEx23-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mCherry interrupted by mdx dystrophin exon 23 bet…

   Promoter

   CAG

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPB-cT3G-cERP2-MYC

   Plasmid

   #192899

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of MYC

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB-cT3G-cERP2-NR2F2

   Plasmid

   #192900

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of NR2F2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   NR2F2 (NR2F2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

   Plasmid

   #134925

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

   Plasmid

   #134922

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

   Plasmid

   #134924

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB-cT3G-cERP2-ZBTB16

   Plasmid

   #192903

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of ZBTB16

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   ZBTB16 (ZBTB16 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPB-cT3G-cERP2-WT1

   Plasmid

   #192906

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of WT1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   WT1 (WT1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid

    #134923

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPB-cT3G-cERP2-PPARG

    Plasmid

    #192912

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of PPARG

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    PPARG (PPARG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPB-cT3G-cERP2-NR1H2

    Plasmid

    #192919

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR1H2 (NR1H2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJOP-HTT-HR18Q

    Plasmid

    #87228

    Purpose

    HTT HR arms with 18 CAG repeats and piggyBac selection cassette

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a driving selection cassette

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB-cT3G-cERP2-ATF4

    Plasmid

    #192911

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of ATF4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-GG-OCT4-1-5-PGK-Puro

    Plasmid

    #102893

    Purpose

    PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid

    #187960

    Purpose

    AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid

    #176459

    Purpose

    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-cT3G-cERP2-ELK1

    Plasmid

    #192918

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of ELK1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OA-874PA

    Plasmid

    #164846

    Purpose

    Expresses AAEL005635-Cas9 with OpIE2-CFP marker

    Depositor

    Omar Akbari

    Article

    Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

    Insert

    P10-3'UTR OpIE2-CFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge