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Plasmid#97105Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmicroRNA 22b Dendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV.UBI.GFP.miR292T
Plasmid#115972PurposemiR-292 regulated GFP expressionDepositorInsertGFP.miR292T
UseLentiviralTagsGFPExpressionMutationPromoterubiquitinAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
R2Ct (1-520)
Plasmid#115649Purposeexpression of truncated Rag2 including the C-terminal regionDepositorInsertRag2 (1-520) (Rag2 Mouse)
UseTags8XHis-MBP-PreScissionExpressionMammalianMutationtruncated Rag2 (aa 1-520)PromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-Metavinculin tail
Plasmid#107152Purposebacterial expressionDepositorInsertmetavinculin (VCL Human)
UseTags(His)8ExpressionBacterialMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgPYM1
Plasmid#105249Purposehuman PYM1 sgRNADepositorInsertsgRNA for PYM1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
P415 δCLD+Ret2 2α
Plasmid#73878PurposeSwapped helicesDepositorInsertδCLD+Ret2 2α
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
P415 Ret2LD+δC2α
Plasmid#73877PurposeSwapped helicesDepositorInsertRet2LD+δC2α
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC S431A T432A NS1
Plasmid#101837Purposedrives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locusDepositorInsertkaiBC S431A T432A with p0050 promoter
UseCyanobacteria cloning vectorTagsExpressionMutationS431A T432APromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC S431E T432E NS1
Plasmid#101836Purposedrives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locusDepositorInsertkaiBC S431E T432E with p0050 promoter
UseCyanobacteria cloning vectorTagsExpressionMutationS431E T432EPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG272-eya
Plasmid#99085PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea eyes absent (eya)DepositorInserteyes absent (eya)
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG318-h.72.4d
Plasmid#99080PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea h.72.4dDepositorInserth.72.4d in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG223-smad2/3
Plasmid#99078PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Smad2/3DepositorInsertSmad2/3 in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutL(R95F)
Plasmid#86608PurposeE. coli Mutl(R95F) expression plasmidDepositorInsertE. coli MutL(R95F)
UseTagshis-tag and ald-tagExpressionBacterialMutationR95FPromoterT7Available sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
TU#1412_tiam-1_cDNA
Plasmid#82364Purposeexpress tiam-1 cDNADepositorInserttiam-1 cDNA (tiam-1 Nematode)
UseTagsExpressionWormMutationPromoternoneAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
TU#1449_unc-73e_cDNA
Plasmid#82363Purposeexpress unc-73e cDNADepositorInsertunc-73 e isoform (unc-73 Nematode)
UseTagsExpressionWormMutationPromoternoneAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW215J105-Amp32C
Plasmid#78650PurposepSB3K3 carrying J105-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ105-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW212J114-Amp32C
Plasmid#78647PurposepSB3K3 carrying J114-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ114-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT7-1 (E1083Q)-intein-CBD
Plasmid#73303PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationE1083QPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD
Plasmid#73299PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationS1079N-E1083QPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA
Plasmid#72703PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AAPromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_LAMP1
Plasmid#65685PurposeExpresses LAMP1-CNL in mammalian cellsDepositorInsertLAMP1
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_LAMP1
Plasmid#65684PurposeExpresses LAMP1-YNL in mammalian cellsDepositorInsertLAMP1
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_PTS2
Plasmid#65675PurposeExpresses PTS2-YNL in mammalian cellsDepositorInsertperoxisomal targeting signal 2
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WTnoGirdles
Plasmid#62653PurposeWild-type OmpA with aromatic girdle residues replaced by leucineDepositorInsertOmpA_noGirdles
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationAromatic girdle residues mutated to Leu (W7, W15,…PromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD-g6
Plasmid#61497PurposeFor expression of C. briggsae gld-1, used for rescue of C. elegans q485 homozygotesDepositorInsertgld-1 (Cbr-gld-1 Caenorhabditis briggsae)
UseTagsFLAG and HisExpressionWormMutationPromotergld1Available sinceFeb. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 100
Plasmid#52989PurposepLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfpDepositorInsertsOxyS sRNA
fhlA
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutation−309 to +96 bp relative to start codonPromoterpLlac0-1 and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET:CACT:GFP
Plasmid#60737PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-GFP-Patronin CC 534–868
Plasmid#59054Purposefor E. coli expression of Patronin CC truncationDepositorInsertPatronin (Patronin Fly)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 802
Plasmid#52991PurposepLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfpDepositorInsertsOxyS sRNA
ompC
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutationcontains −81 to +36 bp relative to start codonPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1148
Plasmid#53006PurposepCon:OxyS, pLlacO-1:sodB::gfpDepositorInsertsOxyS sRNA
sodB
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1070
Plasmid#53002PurposepCon:DsrA, pLlacO-1:sodB::gfpDepositorInsertsDsrA sRNA
sodB
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1057
Plasmid#53001PurposepCon:RhyB, pLlacO-1:rpoS::gfpDepositorInsertsRhyB sRNA
rpoS
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontainsn −150 to +30 bp relative to start codonPromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGMCKq20-T38S38-0X
Plasmid#49522PurposeKanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 siteDepositorInsertconstitutive TetR38
UseTagsExpressionBacterialMutationPromoterPtb38Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Phb
Plasmid#48162Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of Phb (Phb Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Slc25a15 mod
Plasmid#48161Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from elementDepositorInsert5" UTR of Slc25a15
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mrpl1
Plasmid#48166Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of MRPL11 (Mrpl11 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Slc25a15
Plasmid#48160Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of Slc25a15
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
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Torso 11.5 kb rescue fragment
Plasmid#39810DepositorInsertTorso genomic DNA fragment (tor Fly)
UseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 3448 (=HL 3425 + pHL 1191)
Plasmid#30078DepositorInsertpLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 3375 (=HL 865 + pHL 1008)
Plasmid#30072DepositorInsertpLlacO-1:DsrA-mCherry, pLtetO-1:RBS (st7) gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only