Addgene

We narrowed to 3,729 results for: Aes

Showing: 1221 - 1240 of 3729 results

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pCMV-DeAct-SpvB

Plasmid

#89446

Purpose

Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

CMV

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE3G-GSX2

Plasmid

#96964

Purpose

Dox-inducible expression of Gsx2 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 (Gsx2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK1

Plasmid

#138692

Purpose

Expresses a human NUAK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK1 human (NUAK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTpPuc3

Plasmid

#62864

Purpose

Episome vector for Thalassiosira pseudonana (pUC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for thalassios…

Tags

Expression

Mutation

Promoter

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ10715

Plasmid

#183960

Purpose

U6-crOct4-CAG-hyperdCas12a-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

HyperdCas12a
crRNA targeting Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP11-LRRK2_G2019S

Plasmid

#231175

Purpose

Expresses GFP-11-LRRK2 G2019S in mammalian cells

Depositor

Samara Reck-Peterson

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

GFP11-LRRK2 (G2019S) (LRRK2 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

G2019S

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Synaptojanin 1-145

Plasmid

#22291

Depositor

Pietro De Camilli

Article

Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Use

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 E17K

Plasmid

#73050

Purpose

Human AKT3 E17K

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K (AKT3 Human)

Use

Tags

Expression

Mammalian

Mutation

E17K

Promoter

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMDC32B-BS-AtMIR390a-B/c

Plasmid

#199560

Purpose

Plant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stem

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10

Insert

Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)

Use

RNAi

Tags

Expression

Mutation

Promoter

2x35S

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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HT103_FCMV_QuasAr6a_Citrine

Plasmid

#178822

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6a carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6a_Citrine

Use

Lentiviral

Tags

citrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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AID∆C-Cas9n-UGI-NLS

Plasmid

#198890

Purpose

Express AID(1-188) BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Actication induced deaminase (AICDA Human)

Use

Tags

NLS

Expression

Mammalian

Mutation

Deletion of amino acids 189-198

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pVITRO1-Trastuzumab-IgG3/κ

Plasmid

#61885

Purpose

Expresses HER2/neu receptor specific humanized IgG3/κ antibody isotype (Trastuzumab-IgG3/κ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

mEF1 Prom and rEF1 Prom

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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HT109_pAAV_hSyn-DiO-SomQuasAr6a_EGFP

Plasmid

#190878

Purpose

Cre-on expression of soma-targeted QuasAr6a under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

somQuasAr6a_EGFP

Use

AAV

Tags

EGFP

Expression

Mutation

Promoter

hSyn

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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TDG004_pLenti_CMVtight_Kir2.1_CFP

Plasmid

#178820

Purpose

tet-on expression of an inward-rectifier potassium channel Kir2.1 carrying an CFP tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

Kir2.1_CFP

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK2

Plasmid

#138693

Purpose

Expresses a human NUAK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK2 human (NUAK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLI

Plasmid

#131228

Purpose

Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Coding sequence has been codon optimised for expr…

Promoter

CMV enhancer + CMV promoter

Available since

Dec. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Ad-Flag-XBP1s

Plasmid

#63678

Purpose

Adenoviral vector for XBP1s expression

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

spliced

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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AID-NG-CBE

Plasmid

#227416

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AID-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgNT

Plasmid

#138678

Purpose

Expresses a Non-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNT

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG mFoxg1-GFP wildtype

Plasmid

#73048

Purpose

C-terminal fusion of gfp Mouse wildtype Foxg1

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

mFoxg1 (Foxg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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