We narrowed to 3,216 results for: ER;

1. pFsynW SYT1-PGM reporter

   Plasmid

   #197278

   Purpose

   Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 and HaloTag (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

   Promoter

   hSyn (human synapsin I promoter)

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCI pHluorin Syt17

   Plasmid

   #184514

   Purpose

   Mammalian expression of Synaptotagmin 17 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 17 (Syt17 Rat)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCI pHluorin Syt9

   Plasmid

   #184510

   Purpose

   Mammalian expression of Synaptotagmin 9 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 9 (Syt9 Mouse)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCI pHluorin Syt8

   Plasmid

   #184509

   Purpose

   Mammalian expression of Synaptotagmin 8 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 8 (Syt8 Mouse)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCI pHluorin Syt3

   Plasmid

   #184504

   Purpose

   Mammalian expression of Synaptotagmin 3 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 3 (Syt3 Rat)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET30_Halotag_K73T_Jdomain

   Plasmid

   #183693

   Purpose

   Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   ER Halo(K73T)-J domain
   

   Expression

   Bacterial

   Mutation

   K73T in Halotag

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCh-Miro1(∆TM)-SspB

   Plasmid

   #194007

   Purpose

   Optogenetic tool

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dora[B]_rescue_construct

   Plasmid

   #190637

   Purpose

   Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Expression

   Insect

   Mutation

   Changed threonine 295 to methionine

   Promoter

   Actin-5c

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dora[A]_rescue_construct

   Plasmid

   #190639

   Purpose

   Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Expression

   Insect

   Mutation

   Truncated to contain amino acids 1-945

   Promoter

   Actin-5c

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLS.407

    Plasmid

    #187204

    Purpose

    Expresses retron Eco1 ncRNA v32, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v32
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSLS.408

    Plasmid

    #187205

    Purpose

    Expresses retron Eco1 ncRNA v35, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p406 – TDH3-MTS-GFP11-Ura

    Plasmid

    #177733

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA marker

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-GFP11
    

    Expression

    Bacterial and Yeast

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CTAG_eGFP-HDEL

    Plasmid

    #136097

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-HDEL
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CTAG_mTurquoise2-HDEL

    Plasmid

    #136105

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_mTurquoise2(noATG,noStop)-HDEL
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. CTAG_mVenus-HDEL

    Plasmid

    #136107

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_mVenus(noATG,noStop)-HDEL
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGEX-4T Syt1 C2AB(140) M173C

    Plasmid

    #134696

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    M173C, C277A

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T Syt1 C2AB(140) F234C

    Plasmid

    #134697

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F234C, C277A

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-4T Syt1 C2AB(140) V304C

    Plasmid

    #134698

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    V304C, C277A

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T Syt1 C2AB(140) I367C

    Plasmid

    #134699

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    I367C, C277A

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-FLAG-PGRMC-Hybrid1

    Plasmid

    #173469

    Purpose

    N-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge