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LLPS2-CAHS2_PARRC::SspB (pBS1043)

Plasmid

#185290

Purpose

For the mammalian expression of the tardigrade protein CAHS2_PARRC attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2_PARRC

Tags

FLAG

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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LLPS1-FUSN:SspB (pBS1041)

Plasmid

#185288

Purpose

For the mammalian expression of the human protein FUSN attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

FUSN

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7

Plasmid

#180315

Purpose

mammalian expression of human SEPT7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 IGF1R-BirA(R118G)-HA

Plasmid

#109232

Purpose

This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

Depositor

Victor Dzau

Article

Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

Insert

IGF1R (IGF1R Human)

Tags

BirA(R118G) (highly promiscuous form) and HA

Expression

Mammalian

Promoter

T7

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT2-msfGFP

Plasmid

#180318

Purpose

mammalian expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple

Plasmid

#180323

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_mApple-SEPT7 Gmut2

Plasmid

#180317

Purpose

mammalian expression of human SEPT7 Gmut2 fused to mApple

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

mApple

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP10-14-SEPT7

Plasmid

#180341

Purpose

mammalian expression of human SEPT7 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP10

Plasmid

#180343

Purpose

mammalian expression of human SEPT9_i1 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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hTln1-R1R2-17b

Plasmid

#191427

Purpose

Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria

Depositor

Ben Goult

Article

Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

Insert

Human Talin1 R1R2 domains (TLN1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

contains 17AA insertion from inclusion of exon 17b

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP11-14-SEPT7

Plasmid

#180342

Purpose

mammalian expression of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP11

Plasmid

#180344

Purpose

mammalian expression of human SEPT9_i1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP10

Plasmid

#180345

Purpose

mammalian expression of human SEPT9_i3 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP11

Plasmid

#180346

Purpose

mammalian expression of human SEPT9_i3 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut2

Plasmid

#180425

Purpose

mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6

Plasmid

#180313

Purpose

bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN6 (SEPTIN6 Human)

Tags

His6-TEV and sfCherry2

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT2 NCmut-msfGFP

Plasmid

#180320

Purpose

mammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB5_WT

Plasmid

#82898

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

S176P; AA190-192delTDTins25;AA194-196delPVQinsSIP…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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IMPT-10917

Plasmid

#236192

Purpose

Express GPR6 in insect cells

Depositor

Vadim Cherezov

Article

Barekatain et al Sci Signal. 2024 Dec 3;17(865):eado8741.

Insert

GPR6 (GPR6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3 Gmut-msfGFP

Plasmid

#180326

Purpose

mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i3 W502A, H512D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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