We narrowed to 3,220 results for: ER;

1. pGEX-4T Syt1 C2AB(140) V304C

   Plasmid

   #134698

   Purpose

   Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Synaptotagmin 1 (Syt1 Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   V304C, C277A

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T Syt1 C2AB(140) I367C

   Plasmid

   #134699

   Purpose

   Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Synaptotagmin 1 (Syt1 Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   I367C, C277A

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-FLAG-PGRMC-Hybrid1

   Plasmid

   #173469

   Purpose

   N-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-FLAG-PGRMC-Hybrid2

   Plasmid

   #173470

   Purpose

   N-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-FLAG-PGRMC-Hybrid3

   Plasmid

   #173471

   Purpose

   N-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX208-SAE1

   Plasmid

   #171012

   Purpose

   Ectopic expression of SAE1

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   untagged SAE1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX208-nfBFP

   Plasmid

   #171013

   Purpose

   Ectopic expression of nonfluorescent BFP

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   untagged nfBFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   BFP[H66A]

   Promoter

   Ef1-alpha

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLENTI CMV GFP-TREX1 309* BLAST

   Plasmid

   #164242

   Purpose

   lentiviral plasmid for GFP-TREX1 309*

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deletion of amino acids 310-314

   Promoter

   CMV

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLz77_CB_operon_CsoS2_N3

   Plasmid

   #140859

   Purpose

   Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-108 and 220-235 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLz78_CB_operon_CsoS2_delNTD

    Plasmid

    #140860

    Purpose

    Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-235 of CsoS2

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBz172_CsoS2_N1_GFP

    Plasmid

    #140852

    Purpose

    His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 1 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBz175_CsoS2_N4_GFP

    Plasmid

    #140855

    Purpose

    His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 4 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBz173_CsoS2_N2_GFP

    Plasmid

    #140853

    Purpose

    His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 2 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBz174_CsoS2_N3_GFP

    Plasmid

    #140854

    Purpose

    His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 3 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

    Plasmid

    #124066

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-R232Q-sfGFP
    

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    R232Q

    Promoter

    trc

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. refGFP1_pQE30

    Plasmid

    #48644

    Purpose

    Bact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFP

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    refGFP1
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    refGFP C147S, C204Q

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMT-Map7-1783-end

    Plasmid

    #46071

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-1783-end (Map7 Mouse)
    

    Expression

    Bacterial

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMT-Map7-856-1351

    Plasmid

    #46069

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-856-1351 (Map7 Mouse)
    

    Use

    Unspecified

    Expression

    Bacterial

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcUW51-CgE

    Plasmid

    #13762

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

    Insert

    HSV-1 glycoprotein E
    

    Tags

    His

    Expression

    Insect

    Mutation

    C-terminal region of the HSV-1 gE (KOS strain ect…

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pFSW Syt1-C2B V304A,I367A IRES GFP

    Plasmid

    #133821

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Mutation

    V304A, I367A

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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