We narrowed to 12,131 results for: LEA
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Plasmid#79377PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertscaffold
UseCRISPRExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only -
JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)
Plasmid#180468Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorAvailable SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full
Plasmid#79383PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertZhang 2.0 + scaffold
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)
Plasmid#126025Purposeexpress RFP- and mCer-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)
TagsRFPExpressionInsectMutationWTPromoterHsp70Available SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a SVEM hphMX6 lys1-I-SceI cleavage site
Plasmid#67648PurposeI-SceI cleavage site at lys1-hphMX6DepositorInsertsUseTo test i-scei cleavage efficiencyAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin
Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-mEGFP-MBP-TMD(ALA7)
Plasmid#193051PurposeExpression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.DepositorInsertALA7-TMD
TagsIg k-chain leader sequence, MBP, and mEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag
Plasmid#187994PurposeExpresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cellsDepositorInsertmucin2 D1D2D'D3 (MUC2 Human)
Tags6xHistadine followed by TEV cleavage siteExpressionMammalianPromoterCMVAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1D2D' with cleavable His tag
Plasmid#187993PurposeExpresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cellsDepositorInsertMucin2 D1D2D' (MUC2 Human)
Tags6xHistadine followed by TEV cleavage siteExpressionMammalianPromoterCMVAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)
Plasmid#192785PurposeExpression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and mXFPeExpressionMammalianMutationmXFPe: tyrosine 66 to phenylalanine, asparagine 1…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Muc5b D1 (murine) with cleavable His tag
Plasmid#181814PurposeExpresses murine Muc5b D1 with cleavable His tag, residues 50-426DepositorInsertMuc5b D1 (Muc5b Mouse)
TagsTEV cleavage site followed by 6xHistidine tagExpressionMammalianPromoterCMVAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRS-Leader-SARS-CoV-2-EGFP
Plasmid#171585PurposeExpression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporterDepositorInsertTRS-Leader-SARS-CoV-2-d2eGFP
UseLentiviralTags2PEST AND NLSAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber
Plasmid#105806PurposeConcomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-Cerulean; ORF2: TEV-AmberExpressionMammalianAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-mCerulean3-TUBB1A (JDW 1508)
Plasmid#242555PurposeGateway middle entry clone containing mCerulean3-TUBB1A; Cyan fluorescent reporter for visualizing tubulin in cellsDepositorInsertmCerulean3-TUBB1A
UseGateway CloningAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-mCerulean3-TUBB1A (JDW 1367)
Plasmid#224503PurposeGateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cellsDepositorInsertmCerulean3-TUBB1A
UseGateway CloningAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME H2B mCerulean (JDW 1150)
Plasmid#224543PurposeA Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleusDepositorInsertH2B-mCerulean
UseGateway CloningAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; no tag, self cleaving
Plasmid#204813PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
ExpressionBacterialAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-DEST-cjSTELLA-mCerulean-PuroTK
Plasmid#141029PurposeReporter integrationDepositorInsertmCerulean
ExpressionMammalianAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cerulean(1-158)Stop in pcDNA1/amp
Plasmid#60894PurposeThis vector attaches mCerulean(1-158) to the C-terminus of a protein.DepositorInsertCerulean(1-158)Stop
ExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R53A)-Venus
Plasmid#138197PurposeFRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R53A)-Venus (Rapgef4 Synthetic, Mouse)
TagsCerulean and VenusExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only