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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,015 results for: pcDNA3.1+

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Showing: 1241 - 1260 of 10015 results

  4. pcDNA3.1-dCas9-dMQ1-EGFP

    Plasmid
    #89637
    Purpose
    Targeted CpG methylation
    Depositor
    Margaret Goodell
    Publication
    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    site-specific DNA-methyltransferase SssI
    Use
    CRISPR
    Tags
    3*FLAG, 6*His, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    C141S, TGC-->TCC; S317A, AGC-->GCC
    Promoter
    CMV
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    89637-plasmid-map-sequence-id-177705

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  10. pcDNA3.1.Gα12-LgB115

    Plasmid
    #134363
    Purpose
    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gα12-LgB115 (GNA12 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134363-plasmid-map-sequence-id-306384

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  12. pcDNA3.1-CibN-dCas9

    Plasmid
    #60551
    Purpose
    Expresses CibN-dCas9 in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CibN-dCas9
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    60551-plasmid-map-sequence-id-97050

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  20. pcDNA3.1-GGamma13-GFP2

    Plasmid
    #140992
    Purpose
    Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma13-GFP2 (GNG13 Human)
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140992-plasmid-map-sequence-id-302018

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Showing: 1241 - 1260 of 10015 results