We narrowed to 3,953 results for: rela

1. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

   Plasmid

   #80769

   Purpose

   Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Insert

   IFT88 gRNA#1 (IFT88 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. tetO-HEY2

   Plasmid

   #170691

   Purpose

   doxycycline-inducible overexpression of HEY2

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   HEY2 – hes related family bHLH transcription factor with YRPW motif 2 (HEY2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3-EF1A-CCNE1

   Plasmid

   #162553

   Purpose

   Overexpression of CCNE1

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   Overexpression of CCNE1 (CCNE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF1A

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

   Plasmid

   #75082

   Purpose

   An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

   Insert

   Nuc-eYFP
   

   Use

   AAV and Cre/Lox

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330-Pten-2

   Plasmid

   #162552

   Purpose

   sgRNA targeting Pten

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Pten (Pten Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-pGK-nAChRb2(E61C)-IRES-GFP

   Plasmid

   #164777

   Purpose

   cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

   Depositor

   Alexandre Mourot

   Article

   Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

   Insert

   nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   E61C

   Promoter

   pGK

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBdest-GST-PI3Kc1

   Plasmid

   #187992

   Purpose

   Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326

   Depositor

   Sascha Martens

   Inserts

   ATG14 (ATG14 Human)
   VPS34 (PIK3C3 Human)
   VPS15 (PIK3R4 Human)
   BECN1 (BECN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3 Flag Jnk1a1MKK7B2

   Plasmid

   #19731

   Depositor

   Roger Davis

   Article

   Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

   Insert

   Jnk1a1MKK7b2 (Map2k7, MAPK8 Human, Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pIRES2-CMV-nAChRb2(E61C)-IRES-GFP

   Plasmid

   #164779

   Purpose

   cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

   Depositor

   Alexandre Mourot

   Article

   Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

   Insert

   nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E61C

   Promoter

   CMV

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC710 - pAAV EF1a DIO Mem-AcGFP

    Plasmid

    #75081

    Purpose

    An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Mem-AcGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitylation site

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 ATP5O(5’UTR)-SL-FF

    Plasmid

    #85491

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O followed by stem loop (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 ATP5O(TISU)-FF

    Plasmid

    #85489

    Purpose

    Firefly luciferase under the control of ATP5O TISU

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    Full TISU element of ATP5O (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    2nd and 3rd codon of luciferase were modified fro…

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. px330-Apob-2

    Plasmid

    #162549

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. px330-Apob-1

    Plasmid

    #162548

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. px330-Apob-3

    Plasmid

    #162550

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. px330-Apob-4

    Plasmid

    #162551

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xFLAG-DGKδ2-ΔSAMD

    Plasmid

    #226507

    Purpose

    Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene deleted SAM domain

    Depositor

    Fumio Sakane, Chiaki Murakami

    Article

    Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294

    Insert

    DGKD (DGKD Human)
    

    Use

    Tags

    Three tandem FLAG epitope tag and enterokinase cl…

    Expression

    Mammalian

    Mutation

    deleted sterile alpha motif domain (deleted amino…

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. TR01F/hFGFR3-G380R-V5

    Plasmid

    #214908

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR3-G380R variant that is associated with Achondroplasia

    Depositor

    Pavel Krejčí

    Article

    Kimura et al Sci Transl Med. 2021 May 5;13(592):eaba4

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    G380R substitution

    Promoter

    truncated CMV promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. sh2g-ZMYND8-human

    Plasmid

    #201406

    Purpose

    Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

    Insert

    ZMYND8 (ZMYND8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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