165,892 results

1. pPgiCas13b

   Plasmid

   #184833

   Purpose

   Inducible expression of PgiCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Porphyromonas gingivalis
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. tFucci(SA)5

   Plasmid

   #153520

   Purpose

   New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

   Insert

   tFucci(SA)5.
   

   Use

   Tags

   AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dual-sgRNA_hU6-mU6

   Plasmid

   #154194

   Purpose

   Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE9

   Plasmid

   #194208

   Purpose

   Mammalian expression of ABE9

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q/L145T)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2/9n

   Plasmid

   #112865

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCXLE-hOCT3/4-shp53-F

   Plasmid

   #27077

   Purpose

   Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   OCT3/4 (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. kiCAP-AAV-MyoAAV4A

   Plasmid

   #224448

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYNSL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304

   Plasmid

   #25890

   Purpose

   3rd generation lentiviral vector

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-MBP-mSA2

   Plasmid

   #52319

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Use

   Tags

   FLAG, His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPRv2 puro

    Plasmid

    #98290

    Purpose

    Variant of lentiCRISPRv2 that confers puromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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