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MSP809

Plasmid

#98813

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-HPLVGHM-GST

Expression

Bacterial

Mutation

None

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MSP850

Plasmid

#98797

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-HPLVGHM-TEV L1F 111215

Expression

Bacterial

Mutation

None

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MSP851

Plasmid

#98798

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-HPLVGHM-TEV L2F 111215

Expression

Bacterial

Mutation

None

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MSP852

Plasmid

#98799

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-HPLVGHM-TEV L5B 111215

Expression

Bacterial

Mutation

None

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MSP968

Plasmid

#98800

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-ENLYFQM-TEV H28L T30A

Expression

Bacterial

Mutation

H28L, T30A

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEEF1D-FLAG (pcDNA 3.1+) LG6-1

Plasmid

#37365

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pFLAG-EEF1D (pcDNA 3.1+) LG3-1

Plasmid

#37364

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
mCherry-2xCL-YFP-Vpr

Plasmid

#105215

Purpose

Bi-functional Fluorescent reporter protein to visualize HIV-1 fusion and core-trafficking

Depositor

Gregory Melikyan

Article

Sood et al J Biol Chem. 2017 Oct 18. pii: M117.8180

Insert

Vpr

Tags

PC2x (SQNY/IRKVL), eYFP, and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA E45A

Plasmid

#145783

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residue glutamic acid 45 to alanin…

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA M66I

Plasmid

#149687

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residue Met 66 to Iso (CA M66I)

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00409

Plasmid

#34517

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5249 (Blimp-1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pZS039

Plasmid

#134233

Purpose

For in vitro transcription of Miniata CENP-A

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Miniata CENP-A n-terminus (nt 1-204, aa 1-68)

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00471

Plasmid

#39581

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3743 (MTF-1 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

Plasmid

#138524

Purpose

Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

Depositor

Richard Jenner

Article

Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

Insert

PRC2-binding G-tract repeat RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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601-145-8X

Plasmid

#158620

Purpose

DNA fragment production for nucleosome core particle preparation

Depositor

Curt Davey

Article

Vasudevan et al J Mol Biol. 2010 Oct 15;403(1):1-10. doi

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSADuet148

Plasmid

#121910

Purpose

Charge-introduced NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet116

Plasmid

#121915

Purpose

NpuDnaBΔ290ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBΔ290ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSiMPla_N + pSiMPla_C

Plasmid

#134314

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

ampR_gp41-1 N-intein + ampR_gp41-1 C-intein

Expression

Bacterial

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSiMPlh_N + pSiMPlh_C

Plasmid

#134316

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

hygR_gp41-1 N-intein + hygR_gp41-1 C-intein

Expression

Bacterial

Mutation

P254Q in hygR_gp41-1 N-intein

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAL800

Plasmid

#119306

Purpose

precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S

Tags

none

Expression

Bacterial

Mutation

amino acids 1 through 171 deleted, and mutatioms …

Promoter

lac

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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