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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,345 results for: KRAS

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Showing: 1261 - 1280 of 8345 results

  2. pJV128

    Plasmid
    #124252
    Purpose
    In vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Stanley Riddell
    Article
    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92
    Insert
    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Promoter
    T7
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    124252-plasmid-map-sequence-id-242223

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  3. pJV129

    Plasmid
    #124253
    Purpose
    In vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Stanley Riddell
    Article
    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92
    Insert
    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Promoter
    T7
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    124253-plasmid-map-sequence-id-242081

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  7. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid
    #172473
    Purpose
    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.
    Depositor
    Sean R Collins
    Article
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    Use
    Piggybac transposon
    Tags
    polybasic and CAAX motif from Kras, tdKatushka2, …
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172473-plasmid-map-sequence-id-342817

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  13. pCAGGS_m-AKAR5’

    Plasmid
    #114140
    Purpose
    Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5' C-terminally tagged by KRas C tail
    Expression
    Mammalian
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114140-plasmid-map-sequence-id-215469

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  15. pCAGGS_m-AKAR5

    Plasmid
    #114139
    Purpose
    Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 C-terminally tagged by KRas C tail
    Expression
    Mammalian
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114139-plasmid-map-sequence-id-215468

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  18. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

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Showing: 1261 - 1280 of 8345 results