We narrowed to 15,783 results for: LEA

1. pPbuCas13b-gRNA-1

   Plasmid

   #184567

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGWB12_TtNAM-B1

   Plasmid

   #124573

   Purpose

   Expresses full-length TtNAM-B1 with an N-terminal FLAG tag under the 35s promoter.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TaNAM-B1
   

   Use

   Tags

   FLAG

   Expression

   Plant

   Mutation

   Promoter

   35s

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCR8_TtNAM-B1

   Plasmid

   #124575

   Purpose

   TtNAM-B1 CDS in the Gateway-compatible pCR8 vector.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TaNAM-B1
   

   Use

   Topo gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCR8_TaNAM-A1(1-217)

   Plasmid

   #124576

   Purpose

   NAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TaNAM-A1 (1-217)
   

   Use

   Topo gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCR8_TtNAM-B1(1-215)

   Plasmid

   #124577

   Purpose

   NAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 vector.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TtNAM-B1 (1-215)
   

   Use

   Topo gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT3TS-Hypa-nCas9n-A69

   Plasmid

   #125645

   Purpose

   micro-injection of poly-adenylated Hypa-nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   Hypa-nCas9n
   

   Use

   Rna in vitro transcription vector

   Tags

   Expression

   Mutation

   N692A, M694A, Q695A, H698A from wild-type Cas9

   Promoter

   T3

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOCC176

   Plasmid

   #118891

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6-MBP, cleavable with 3C protease; SNAP, cleav…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pF(UG) hSyn NS34a-msGFP-A'Q-mito

   Plasmid

   #158766

   Purpose

   Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Flexible linkers between the protease and msGFP a…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pF(UG) hSyn NS34a-msGFP-'Q-mito

   Plasmid

   #158767

   Purpose

   Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Flexible linkers between the protease and msGFP a…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. polyQ74-GFP

    Plasmid

    #231893

    Purpose

    Forms cytosolic HTT partial exon 1 Q74 aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICE-HA-MRE11-H129N

    Plasmid

    #82034

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Nuclease-dead mutant: H129N MRE11

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICE-HA-MRE11-H63D

    Plasmid

    #82036

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Exonuclease-dead mutant: H63D MRE11

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

    Plasmid

    #167295

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAK (BAK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

    Plasmid

    #167296

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAX (BAX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-polyQ23-NLS

    Plasmid

    #231891

    Purpose

    Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q23-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDsRed1-CMV-Rat Arc-DsRed1-pA

    Plasmid

    #233049

    Purpose

    To Express a Rat Arc-DsRed fusion protein from a CMV promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Neurobiol Learn Mem. 2015 Oct;124:34-47.

    Insert

    rat Arc-DsRed fusion (Arc Rat)
    

    Use

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

    Plasmid

    #177849

    Purpose

    Bacterial Expression of PNR

    Depositor

    Karsten Melcher

    Article

    Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

    Insert

    photoreceptor cell-specific nuclear receptor (NR2E3 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+-FLAG3-drAgo2DY-EF

    Plasmid

    #221716

    Purpose

    Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2DY-EF (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    D651E and Y680F in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-sACt-mCherry-NES

    Plasmid

    #138215

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NES (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge