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pET22b-ecDHFR

Plasmid

#109055

Purpose

Bacterial expression of E.coli DHFR protein

Depositor

Stephen Benkovic

Article

Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

Insert

ecDHFR

Expression

Bacterial

Promoter

T7

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-EOS-GFP-IP

Plasmid

#60439

Purpose

PiggyBac for EOS-GFP

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

EOS

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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plvx-tetO-GFP

Plasmid

#191560

Purpose

Inducible expression of GFP

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

Insert

gfp

Use

Lentiviral

Mutation

None

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-QUASM1

Plasmid

#155114

Purpose

5' entry vector containing QUASM1 promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1

Use

Gateway 5' entry vector

Promoter

QUASM1-E1b

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMslc

Plasmid

#32095

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

SLC35A1 (SLC35A1 Human)

Expression

Mammalian

Promoter

FerH

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pET14-Encap

Plasmid

#86405

Purpose

Expresses T. maritima encapsulin in E. coli

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Insert

Encapsulin

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pABY-c15

Plasmid

#201768

Purpose

Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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CP2-TCC

Plasmid

#179679

Purpose

Complementary plasmid

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd2] T7 RNAP-TCC linker-degron

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAG244 iFAST

Plasmid

#130809

Purpose

Expresses iFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Trip-GFP-hOCLN

Plasmid

#32789

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-ECFPEYFP

Plasmid

#24520

Depositor

Liusheng He, Jehonathan Pinthus

Article

He et al Cytometry A. 2003 Oct . 55(2):71-85.

Insert

ECFP, EYFP

Tags

ECFP and EYFP

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pYFJ84

Plasmid

#227644

Purpose

Vibrio harveyi acyl-ACP synthetase

Depositor

John Cronan

Article

Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

Insert

Acyl-Acyl carrier protein synthetase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJC175e-DddI

Plasmid

#179693

Purpose

Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[SD8] gIII

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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6x Halo-EGFP- 2x PACT

Plasmid

#107265

Purpose

6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

6x Halo-EGFP- 2x PACT

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_CreERT2

Plasmid

#62427

Purpose

MXS_chaining vector with CreERT2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tamoxifen inducible Cre recombinase

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b-PunyEEER

Plasmid

#208322

Purpose

Bacterial expression of the His-tagged peptide PunyEEER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PunyERRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TinyERRE

Plasmid

#208320

Purpose

Bacterial expression of the His-tagged peptide TinyERRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TinyERRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pETDuet1_CoiC_CoiSA

Plasmid

#208762

Purpose

Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiC
CoiSA

Expression

Bacterial

Mutation

single nucleotide insertion (C70) at RBS of CoiC

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-S577A

Plasmid

#92235

Purpose

Express S577A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-S577A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Serine 577 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Expression

Plant

Mutation

D156R

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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