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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,507 results for: SPI

Showing: 1281 - 1300 of 5507 results

  5. p5E-EFS (EF1a core) (JDW 1319)

    Plasmid
    #224474
    Purpose
    Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    EFS promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    224474-plasmid-map-sequence-id-447208

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  14. pCMV_NFL(K468TAG)-FLAG

    Plasmid
    #182658
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K468 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K468TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182658-plasmid-map-sequence-id-363090

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  15. pCMV_NFL(K211TAG)-FLAG

    Plasmid
    #182655
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K211 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K211TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182655-plasmid-map-sequence-id-363059

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  16. pCMV_NFL(R438TAG)-FLAG

    Plasmid
    #182657
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position R438 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    R438TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182657-plasmid-map-sequence-id-363055

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  17. pORANGE_NFL linker-3xFLAG KI

    Plasmid
    #182679
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182679-plasmid-map-sequence-id-363017

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  18. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid
    #182680
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182680-plasmid-map-sequence-id-363164

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  19. pCMV_FLAG-NFL(K468TAG)

    Plasmid
    #182663
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K468 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K468TAG
    Promoter
    CMV
    Available since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182663-plasmid-map-sequence-id-362921

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  20. pET24a-VHH-tev-2xTS

    Plasmid
    #117750
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    117750-plasmid-map-sequence-id-228351

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Showing: 1281 - 1300 of 5507 results