Addgene

We narrowed to 4,659 results for: pcr

Showing: 1281 - 1300 of 4659 results

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TAL-1GT2

Plasmid

#41455

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

1GT2

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-1TA2

Plasmid

#41456

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

1TA2

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-1TT2

Plasmid

#41459

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

1TT2

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-3AA4

Plasmid

#41460

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

3AA4

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-3AC4

Plasmid

#41461

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

3AC4

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-3GA4

Plasmid

#41468

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

3GA4

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pPOTv7-blast-mNG

Plasmid

#179817

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3Myc

Plasmid

#221046

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xMyc epitopes

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMK782 Septin6-GFP

Plasmid

#38296

Depositor

Matthew Krummel

Article

Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Insert

Septin 6 (Septin6 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

22 amino acid linker sequence (added by PCR)

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-CLIPtag

Plasmid

#179787

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

CLIP

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

Plasmid

#126049

Purpose

For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-natMX6 (pFB1434)

Plasmid

#126050

Purpose

For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA

Plasmid

#174030

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-Venus-PEST

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-TgKI-MCS-eGFP-MCS-polyA

Plasmid

#174024

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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M-NM-sgRNA

Plasmid

#48673

Purpose

Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pPOTv7.1-phleo-mNG

Plasmid

#181935

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJS001

Plasmid

#188324

Purpose

PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::GFP::Linker

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-His:mNG:His

Plasmid

#201094

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJS012

Plasmid

#188335

Purpose

PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mTagBFP2::mIAA7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

Plasmid

#174028

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-eGFP

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge