We narrowed to 7,091 results for: plasmid dna

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(81) pcDNA3.1-BC2-Nup62-Flag-EPEA

Plasmid

#168094

Purpose

Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

Nup62-Ubc-Flag-EPEA

Tags

BC2-Tag, EPEA-tag, and Flag-tag

Expression

Mammalian

Mutation

P229A, S283T on Nup62 native sequence

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-mir17~20

Plasmid

#46671

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir17~20 (MIR17 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-6His-mEsrra

Plasmid

#173152

Purpose

mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

Depositor

Brijesh Kumar Singh

Article

Tripathi et al bioRxiv 2021.11.16.468891

Insert

ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)

Tags

His

Expression

Mammalian

Mutation

Wild type

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(5’UTR)-FF

Plasmid

#85486

Purpose

Firefly luciferase under the control of ATP5O 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of ATP5O (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(5’UTR)-SL-FF

Plasmid

#85491

Purpose

Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of ATP5O followed by stem loop (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(TISU)-FF

Plasmid

#85489

Purpose

Firefly luciferase under the control of ATP5O TISU

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

Full TISU element of ATP5O (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

2nd and 3rd codon of luciferase were modified fro…

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 UQCC2(5’UTR)-FF

Plasmid

#85487

Purpose

Firefly luciferase under the control of UQCC2 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of UQCC2 (C6orf125 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD]

Plasmid

#190086

Purpose

express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Tags

Rhodopsin C9

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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(112) pcDNA3.1-GFP-Flag-Nup62-EPEA

Plasmid

#168101

Purpose

GFP and Flag tagged Nup62 with EPEA nanobody tag

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

GFP-Flag-Nup62-EPEA

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

Plasmid

#125796

Purpose

for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CD40L (lose of function mutant)-mgSrtA

Expression

Mammalian

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

Plasmid

#72888

Purpose

fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

Insert

Gaussia luciferase, Channelrhodopsin-2, EYFP

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

Plasmid

#168189

Purpose

HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter dme-mir-125

Plasmid

#46667

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-125

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)

Plasmid

#72889

Purpose

fusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO2) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

Inserts

Gaussia luciferase, Volvox channelrhodopsin 1, EYFP
Volvox channelrhodopsin 1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter dme-mir-279

Plasmid

#46668

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-279

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter dme-mir-92a

Plasmid

#46666

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-92a

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

Plasmid

#225587

Purpose

Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Article

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

T21R-vhhGFP4-T2A-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

Plasmid

#157693

Purpose

mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.2 mir-1-1 reporter mir16-1.2

Plasmid

#46681

Purpose

Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1.2 (MIR16-1 Synthetic, Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 T21R-Nb139-T2A-mCherry

Plasmid

#225589

Purpose

Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Article

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

T21R-Nb139-T2A-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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