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pCFJ326 - operon for germline expression

Plasmid

#34875

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

tbb-2 gld-1 operon

Use

Worm targeting

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCM5.48

Plasmid

#19385

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

mex-5 3'utr in pDONRP2RP3 (mex-5 Nematode)

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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L4440-daf-16af

Plasmid

#31506

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a/f common (daf-16 Nematode)

Use

RNAi

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCM5.50

Plasmid

#19383

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

spn-4 3'utr in pDONRP2RP3 (spn-4 Nematode)

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pET_Unc653_GFP_His

Plasmid

#14107

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (1-653) (unc-104 Nematode)

Tags

GFP and His

Expression

Bacterial

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pCG26

Plasmid

#26938

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

dcap-1 (dcap-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only
sgRNA with rpr-1 promoter

Plasmid

#48961

Purpose

to drive the sgRNA expression under RNase P non-coding RNA promoter

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

rpr-1 promoter

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAP693-3

Plasmid

#99492

Purpose

nirFP::3xFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

nirFP::3xFlag

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM-GluCl_cryst

Plasmid

#31487

Depositor

Eric Gouaux

Article

Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

Insert

C. elegans glutamate-gated chloride channel alpha, crystallized construct

Use

For rna synthesis for expression in x. laevis ooc…

Tags

8x-His

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pOX-twk18

Plasmid

#16430

Depositor

Larry Salkoff

Article

Kunkel et al J Neurosci. 2000 Oct 15. 20(20):7517-24.

Insert

Tandem pore domain K+ channel twk-18 (twk-18 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pCFJ448 - pDESTR4-R3(ttTi4348, I, unc-18)

Plasmid

#34865

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4348 targeting

Use

Worm targeting

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pJRK254

Plasmid

#173742

Purpose

donor plasmid for SapTrap assembly

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

VHH(mCherry)

Use

Other

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK112

Plasmid

#173735

Purpose

donor plasmid for SapTrap assembly

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

VHH(GFP)

Use

Other

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCM5.46

Plasmid

#19386

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pgl-3 3'utr in pDONRP2RP3 (pgl-3 Nematode)

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pEV9.03

Plasmid

#37495

Depositor

Geraldine Seydoux

Article

Voronina et al Development. 2010 May;137(9):1441-50. Ep

Insert

npp-16 (npp-16 Nematode)

Use

RNAi

Expression

Worm

Mutation

nts 162-673 of cds

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pET28b-Ava3856

Plasmid

#45742

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3856 (Ava_3856 Anabaena variabilis)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pCZ47

Plasmid

#128414

Purpose

vab-1 rescuing minigene

Depositor

Andrew Chisholm

Article

George et al Cell. 1998 Mar 6;92(5):633-43.

Insert

vab-1 (vab-1 Nematode)

Use

Unspecified

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCM5.40

Plasmid

#19393

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

glp-1 3'utr in pDONRP2RP3 (glp-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pRB1084

Plasmid

#71516

Purpose

Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 mutant

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pdestRG5273Neo

Plasmid

#26390

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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