We narrowed to 9,951 results for: pET

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pScPPX2

Plasmid

#112877

Purpose

Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

Depositor

Michael Gray, Ursula Jakob

Publication

Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

Insert

PPX1 (PPX1 Budding Yeast)

Tags

6X His tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a(+) NRP1 b1b2 (273-586)

Plasmid

#162733

Purpose

Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Publication

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1b2 domains (residues 273-586)

Tags

6His

Expression

Bacterial

Mutation

Codon optimised

Available since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET_3a_Ub_ENT

Plasmid

#237412

Purpose

Expresses Ubiquitin with a disordered tail from the extreme N terminus of Rpn13

Depositor

Kylie Walters

Publication

Hassan et al Nat Commun. 2025 Jul 1;16(1):5669. doi:

Insert

Ubiquitin_ENT

Expression

Bacterial

Promoter

T7

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pET_3a_Ub_EDGEGEE

Plasmid

#237413

Purpose

Expresses Ubiquitin with a disordered tail with an acidic sequence

Depositor

Kylie Walters

Publication

Hassan et al Nat Commun. 2025 Jul 1;16(1):5669. doi:

Insert

Ubiquitin_EDGEGEE

Expression

Bacterial

Promoter

T7

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCAD183_gCAD_pET

Plasmid

#239119

Purpose

Gorilla ACOD1 in E.coli expression vector

Depositor

Konrad Buessow

Publication

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

ACOD1

Tags

StrepTag-II and TEV site

Expression

Bacterial

Promoter

T7

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET-PC(1-86)-H6

Plasmid

#183780

Purpose

Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

Depositor

Peter Harte

Publication

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Tags

6 His Tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET_4E1H.95

Plasmid

#155213

Purpose

Expresses 4E1H.95 de novo protein in E.coli

Depositor

Bruno Correia

Publication

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95

Tags

6x His Tag

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4H.01

Plasmid

#155210

Purpose

Expresses 4H.01 de novo designed protein in E.coli

Depositor

Bruno Correia

Publication

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4H.01

Tags

6x His Tag

Expression

Bacterial

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3E2H.37

Plasmid

#155211

Purpose

Expresses 3E2H.37 de novo designed protein in E.coli

Depositor

Bruno Correia

Publication

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3E2H.37

Tags

6x His Tag

Expression

Bacterial

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3H1L_02.395

Plasmid

#155198

Purpose

Expresses 3H1L_02.395 in E.coli

Depositor

Bruno Correia

Publication

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3H1L_02.395

Tags

6x His Tag

Expression

Bacterial

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET:CACT:GFP

Plasmid

#60737

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.

Depositor

Matthew Bennett

Publication

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

Plasmid

#48311

Depositor

Scott Gradia

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET30-2-PSAT1

Plasmid

#83908

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine aminotransferase 1 (PSAT1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His-mEGFP-iLID

Plasmid

#246940

Purpose

Expresses His-mEGFP-iLID in E.coli

Depositor

Makito Miyazaki

Publication

Yamamoto et al Nat Commun. 2025 Aug 26;16(1):7583. doi:

Insert

iLID

Tags

His-tag, mEGFP

Expression

Bacterial

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(WT)

Plasmid

#245191

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Publication

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

wild-type

Available since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP14-SARS-CoV-2

Plasmid

#236423

Purpose

bacterial protein expression of NSP14

Depositor

Michele Pagano

Publication

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP14 (ORF1ab SARS-CoV-2)

Tags

6xHis

Expression

Bacterial

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA RBS30 mscarlet

Plasmid

#226370

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

Depositor

Urartu Seker

Publication

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

mScarlet

Tags

6x Histag

Expression

Bacterial

Promoter

copA promoter (pCopA)

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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PET-28a-YTH domain of YTHDC2

Plasmid

#228779

Purpose

Protein expression plasmid for YTH domain of YTHDC2 in E.Coli.

Depositor

Huiqing Zhou

Publication

Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

Insert

YTH domain of YTHDC2 (YTHDC2 Human)

Tags

6*His

Expression

Bacterial

Promoter

T7

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-Sniper SpCas9-NLS-6xHis

Plasmid

#207374

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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