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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,839 results for: pcr

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Showing: 1301 - 1320 of 4839 results

  4. pADH147

    Plasmid
    #90991
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.
    Depositor
    Aaron Hernday
    Publication
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90991-plasmid-map-sequence-id-180524

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  5. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  9. pAG25-L-IFP-F[1] (clon 3)

    Plasmid
    #52899
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Publication
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    L-IFP-F[1]
    Use
    Vector for homologous recombination
    Promoter
    TEF promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pAG32-L-IFP-F[2] (clon 3)

    Plasmid
    #52900
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Publication
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    L-IFP-F[2]
    Use
    Vector for homologous recombination
    Promoter
    TEF promoter
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Kiran Musunuru
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    64710-plasmid-map-sequence-id-108923

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Showing: 1301 - 1320 of 4839 results