We narrowed to 4,163 results for: spl

Showing: 1301 - 1320 of 4163 results

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pTopo pLox hPGK-Puro pA pLox

Plasmid

#171048

Purpose

Empty donor template for CRISPR/Cas9 targeting of TERT RE's

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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PYL1_nPPVp

Plasmid

#119211

Purpose

Expresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nPPVp in fusion with PYL1

Tags

AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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FRB_nPPVp

Plasmid

#118966

Purpose

Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nPPVp in fusion with FRB

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PLL7.0 tgRFP cPYP

Plasmid

#114464

Purpose

Mammalian expression of tgRFP labelled cPYP

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

cPYP

Tags

tgRFP

Expression

Mammalian

Mutation

M121E

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA RBD-GFP11

Plasmid

#133069

Purpose

Expresses RBD domain with tripartite split GFP11 (M4)

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

RBD (Rhotekin-Rho Binding Domain)

Tags

GFP11 (tripartite split-GFP)

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-hSyn-IntCCCre-T2A-CopGFP

Plasmid

#160503

Purpose

Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

IntCCCre

Use

Cre/Lox and Lentiviral

Tags

CopGFP

Expression

Mammalian

Promoter

hSyn

Available Since

March 12, 2022

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_N

Plasmid

#209553

Purpose

pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

TadA-8e(2-76)-2xFRB

Expression

Mammalian

Promoter

pCMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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SPDK3181

Plasmid

#97403

Purpose

binary construct of ER-targeted sfCherry1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-sfCherry1-10

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-3TM-tevs-KKMP

Plasmid

#179634

Purpose

TEVP-inducible tri-transmembrane SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-SdB-T

Plasmid

#87307

Purpose

Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Stabilized dimeric Broccoli aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)EGFP/CMV/WPRE∆U3

Plasmid

#41790

Purpose

Lentiviral vector for CMV promoter driven expression of EGFP

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

EGFP

Use

Lentiviral

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-GST-hSYK

Plasmid

#211892

Purpose

Express human SYK in mammalian cells

Depositor

Haian Fu

Insert

SYK (SYK Human)

Tags

GST tag

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDragon-Ctgf

Plasmid

#155015

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

Ccn2 (Ccn2 Mouse)

Use

Mouse Targeting

Mutation

M24T (please see depositor comments)

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDragon-p21

Plasmid

#155016

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activity

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

Cdkn1a (Cdkn1a Mouse)

Use

Mouse Targeting

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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mTB3-BC2 nanobody

Plasmid

#210208

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

BC2 Nanobody

Expression

Bacterial

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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PEP107E

Plasmid

#97393

Purpose

binary construct of Golgi-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Golgi target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PEP111E

Plasmid

#97397

Purpose

binary construct of plastid-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Plastid target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hSeparase

Plasmid

#33017

Depositor

Marc Kirschner

Article

Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Insert

Separase (ESPL1 Human)

Tags

6xMyc

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only