We narrowed to 4,215 results for: spl

Showing: 1301 - 1320 of 4215 results

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pDragon-DTA

Plasmid

#155017

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to attenuated diphtheria toxin (tox176) in the presence of Cre and (r)tTA activity

Depositor

Alberto Rosello-Diez

Publication

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

DTA

Use

Mouse Targeting

Mutation

tox176

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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PE_CO-Mini-C

Plasmid

#190337

Purpose

Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion

Depositor

Rasmus Bak

Publication

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE_CO-Mini-C, PCSK9 +1A insertion pegRNA

Use

AAV

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-tBid-pEGFP-C1

Plasmid

#166736

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid

Depositor

David Andrews

Publication

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

tBid (Bid Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Rtn2b-GFP

Plasmid

#186599

Purpose

Encodes human full-length Reticulon 2 isoform B fused to GFP

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-mCherry

Plasmid

#186600

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mCherry

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pJSM103

Plasmid

#48872

Purpose

NHEJ reporter plasmid for blunt ends

Depositor

Michael Glickman

Publication

Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

Insert

split lacZ

Expression

Bacterial

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-D2irG4kwh

Plasmid

#44723

Depositor

Gabor Balazsi

Publication

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
EGFP

Use

Synthetic Biology; Expression regulator/reporter

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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FKBP_cPPVp

Plasmid

#118967

Purpose

Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

Depositor

Roman Jerala

Publication

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cPPVp in fusion with FKBP

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1815_pCR8-gSMN2-DN3

Plasmid

#118648

Purpose

Transient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRAM29

Plasmid

#234315

Purpose

G264A Cat-RNA U1367, Catalytically dead ribozyme control

Depositor

James Chappell

Publication

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

Splicing-deficient mutant of ribozyme

Use

Synthetic Biology

Promoter

P. CymRC

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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ABI_cPPVp

Plasmid

#119212

Purpose

Expresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domain

Depositor

Roman Jerala

Publication

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cPPVp in fusion with ABI

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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sZF4

Plasmid

#43835

Depositor

George Church

Publication

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF4

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pMMDuet19

Plasmid

#45594

Depositor

Hideo Iwai

Publication

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

NpuDnaB mini intein

Tags

GB1 and H6-GB1

Expression

Bacterial

Mutation

removal of endonuclease domain

Promoter

T7

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

Plasmid

#229770

Purpose

Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

Depositor

Hirofumi Kai

Publication

Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

Insert

Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)

Use

Luciferase

Tags

LgBiT

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

Plasmid

#229769

Purpose

Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

Depositor

Hirofumi Kai

Publication

Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

Insert

Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)

Use

Luciferase

Tags

SmBiT

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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P2_HNF4A8

Plasmid

#31094

Depositor

Gerhart Ryffel

Publication

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 8 derived from promoter P2

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMV-dCasRx-ccdB

Plasmid

#221003

Purpose

Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

Depositor

Benjamin Blencowe, Mikko Taipale

Publication

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_C

Plasmid

#209554

Purpose

pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

Depositor

Xue Gao

Publication

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

2xFKBP-TadA-8e(77-167)-SpCas9(D10A)

Expression

Mammalian

Promoter

pCMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTopo pLox hPGK-Puro pA pLox

Plasmid

#171048

Purpose

Empty donor template for CRISPR/Cas9 targeting of TERT RE's

Depositor

Agnel Sfeir

Publication

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PYL1_nPPVp

Plasmid

#119211

Purpose

Expresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domain

Depositor

Roman Jerala

Publication

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nPPVp in fusion with PYL1

Tags

AU1 tag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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