We narrowed to 2,568 results for: CCS

1. pCKC775

   Plasmid

   #226626

   Purpose

   piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
   EF1a promoter, sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   7xTCF-LEF and EF1a

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCKC764

   Plasmid

   #226625

   Purpose

   piggyBAC pCAG-hCas9-T2A-Unbiased3

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, S. pyogenes, Chicken)
   EF1a promoter, blasticidin resistance
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and EF1a

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCKC776

   Plasmid

   #226627

   Purpose

   piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
   EF1a promoter, mCherry
   

   Use

   Synthetic Biology

   Tags

   oxygen dependent degron (ODD)

   Expression

   Mammalian

   Mutation

   Promoter

   4xHRE_YB TATA and EF1a

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_TRE3G_hRTN3E-Halo

   Plasmid

   #216252

   Purpose

   Tet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vector

   Depositor

   Edward Avezov

   Article

   Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

   Insert

   reticulon-3 isoform e (RTN3 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA1_Cas9-hGem_Smc4

   Plasmid

   #217667

   Purpose

   expresses Cas9-hGem and guideRNA for tagging of SMC4

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHDR_NPM2-T2A-mGreenLantern-PuroTK

   Plasmid

   #222910

   Purpose

   Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mGreenLantern
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHDR_FIGLA-T2A-mGreenLantern-PuroTK

   Plasmid

   #222905

   Purpose

   Homology directed repair template for knocking in mGreenLantern reporter to FIGLA.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mGreenLantern
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-SOX17

   Plasmid

   #222925

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of SOX17

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-PRDM1

   Plasmid

   #222924

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   PRDM1 (PRDM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-cT3G-cERP2-ZGLP1

    Plasmid

    #222926

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    ZGLP1 (ZGLP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-cT3G-cERH-SOHLH1

    Plasmid

    #222922

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    SOHLH1 (SOHLH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Hygro-P2A-HA-FKBP-WAPL

    Plasmid

    #217666

    Purpose

    Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    WapI (WAPL Human)
    

    Use

    Tags

    dTag (FKBP36V protein)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Lac

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. WT_IFL_G1A-T2A_R

    Plasmid

    #200183

    Purpose

    WT PA14 I-F Leader and G1A-T2A Repeat

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G1A-T2A in the I-F repeat

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. WT_IFL_G1A-T2A-A28C_R

    Plasmid

    #200185

    Purpose

    WT PA14 I-F Leader and G1A-T2A-A28C Repeat

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G1A-T2A-A28C in the I-F repeat

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRAW-457

    Plasmid

    #200213

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas1 H25A mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRAW-458

    Plasmid

    #200214

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas1 E184A mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRAW-459

    Plasmid

    #200215

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas1 K214A mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRAW-460

    Plasmid

    #200216

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 R55E-N56D mutations

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRAW-461

    Plasmid

    #200217

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E mutations

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRAW-462

    Plasmid

    #200218

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Use

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E, R55E-N56D mutations

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge