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Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
UseTagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.0
Plasmid#32447PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)DepositorInsertG-GECO1.0
UseTagsExpressionMammalianMutationGCaMP3 K119I/L173Q/S404G/E430VPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-PGK-EiP
Plasmid#21312PurposeControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.1
Plasmid#32445PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)DepositorInsertG-GECO1.1
UseTagsExpressionMammalianMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
PBMN YFP-ER50
Plasmid#37261DepositorInsertER50 (ESR1 Human)
UseRetroviralTagsHA-YFPExpressionMutation6 mutations, T371A, L384M, M421G, G521R, Y537S, N…PromoterLTRAvailable sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBMN ER50-YFP
Plasmid#37259DepositorInsertER50 (ESR1 Human)
UseRetroviralTagsYFP-HAExpressionMutation6 mutations, T371A, L384M, M421G, G521R, Y537S, N…PromoterLTRAvailable sinceAug. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro
Plasmid#31480DepositorInsertTraffic Light Reporter 1.1 (ani target)
UseLentiviralTagsExpressionMutationmCherry has M9S, M16L to reduce background mCherr…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Neo/pTER shEGFP#2 (w22-2)
Plasmid#17480Purpose3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, NeoDepositorInsertEnhanced green fluorescent protein shRNA #2
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta11 Aphrodite
Plasmid#13569DepositorInsertFLIPglu F16A Delta11 Aphrodite
UseTagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Neo/pTER shEGFP#1 (w21-2)
Plasmid#17479Purpose3rd gen lentiviral eGFP shRNA #1 (H1/TO promoter), 3’LTR insertion, NeoDepositorInsertEnhanced green fluorescent protein shRNA #1
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta11 Ares-Aphrodite
Plasmid#13571DepositorInsertFLIPglu F16A Delta11 Aphrodite
UseTagsAphrodite and AresExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta13 Aphrodite
Plasmid#13570DepositorInsertFLIPglu F16A Delta13 Aphrodite
UseTagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHL1670
Plasmid#37634DepositorInsertsUseSynthetic BiologyTagsASV degradation tag and glgC leader regionExpressionBacterialMutationPromoterpConM12NoHind and pConNoHindAvailable sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL1668
Plasmid#37633DepositorInsertsUseSynthetic BiologyTagsASV degradation tag and glgC leader regionExpressionBacterialMutationPromoterpConM12NoHind and pConNoHindAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL1530
Plasmid#37569DepositorInsertsGFP
TetR
CsrA
UseSynthetic BiologyTagsglgC leader regionExpressionBacterialMutationstart codon removedPromoterpConNoHind and pLlacO-1Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL1355
Plasmid#37566DepositorInsertsGFP
TetR
CsrA
UseSynthetic BiologyTagsglgC leader regionExpressionBacterialMutationstart codon removedPromoterpConNoHind and pConNoHindM12Available sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP
Plasmid#26966PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibitionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationER export at 3' end of gene. Trafficking sig…PromoterEF1alphaAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-IRES-RFP
Plasmid#35395DepositorInsertsUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-IRES-GFP
Plasmid#35396DepositorInsertsUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-GTB
Plasmid#26197DepositorInsertEnhanced green fluorescent protein, TVA, rabies B19 glycoprotein
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationTVA, rabies glycoprotein are mammalian codon opti…PromoterAvailable sinceNov. 11, 2010AvailabilityAcademic Institutions and Nonprofits only