We narrowed to 1,469 results for: MiCy

1. pAcBac1-Mb-sfGFP 150TAG

   Plasmid

   #197570

   Purpose

   Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   Pyl-tRNA (4 copies)
   

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   N150TAG

   Promoter

   CMV and U6/H1

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOmnibac_ZZ_linker_K963_GFP_SNAP

   Plasmid

   #159727

   Purpose

   Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
   

   Tags

   GFP and SNAP tags and ZZ affinity tag

   Expression

   Insect

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOmnibac_ZZ_linker_ybbR_Tau

   Plasmid

   #159717

   Purpose

   Expresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   Microtubule-associated protein tau (MAPT Human)
   

   Tags

   ZZ affinity tag, ybbR tag

   Expression

   Insect

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

   Plasmid

   #169182

   Purpose

   Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Tags

   His6-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBFC0687

   Plasmid

   #186692

   Purpose

   ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsB
   tnsC
   tniQ
   Cas12k
   Sh_2xBsaI_NT (Guide Stuffer) gRNA
   Tn7 transposon
   GmR+sfGFP ShDART Cargo
   

   Expression

   Bacterial

   Promoter

   PLac

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR207 SARS-CoV-2 E_nostop

   Plasmid

   #149321

   Purpose

   Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   E (E SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDONR207 SARS-CoV-2 NSP1_nostop

   Plasmid

   #149304

   Purpose

   Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBIG1b_10xHisTEVATG5_ATG12

   Plasmid

   #190861

   Purpose

   Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG12 (ATG12 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial and Insect

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR207 SARS-CoV2 TEV-NSP3

   Plasmid

   #154402

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Sept. 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid

    #111573

    Purpose

    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    lysine to glutamine mutation at the following ami…

    Promoter

    polyhedrin promoter

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR207 SARS-CoV-2 ORF3A_nostop

    Plasmid

    #149319

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDONR207 SARS-CoV-2 M_nostop

    Plasmid

    #149322

    Purpose

    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pFl Strep-sumo-TEV-hAgo2 D597N

    Plasmid

    #136236

    Purpose

    Expressing catalytically inactive human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Tags

    Sterp Sumo

    Expression

    Insect

    Mutation

    D597N

    Promoter

    polyhedrin

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid

    #217897

    Purpose

    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    

    Tags

    2xSTII

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

    Plasmid

    #118412

    Purpose

    Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

    Depositor

    Nicole Paulk

    Article

    Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

    Insert

    Firefly luciferase
    

    Use

    AAV, Luciferase, and Synthetic Biology

    Expression

    Insect and Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR207 SARS-CoV-2 ORF7A_nostop

    Plasmid

    #149324

    Purpose

    Gateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pOmnibac_ZZ_linker_K560_GFP_SNAP

    Plasmid

    #159720

    Purpose

    Expresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    KIF5B (only amino acids 1-560), K560 (KIF5B Human)
    

    Tags

    GFP and SNAP tags and ZZ affinity tag

    Expression

    Insect

    Mutation

    Only amino acids 1-560

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBFC0694

    Plasmid

    #186693

    Purpose

    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_lacZ_α_1 gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR207 SARS-CoV-2 E 27nt-del

    Plasmid

    #153954

    Purpose

    Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    June 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pLIB_GST-nsp13 (SARS-CoV-2)

    Plasmid

    #169190

    Purpose

    Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    GST-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    GST

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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