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pX330-EN1082_Rad21_STOP

Plasmid

#156450

Purpose

For transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codon

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

Cas9, RAD21 (Rad21 Mouse)

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-FRBx5-His

Plasmid

#103784

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

CFP-FRBx5-His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_ER-RE_MLPmin_BC1065-luc2

Plasmid

#227111

Purpose

Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-mir124a

Plasmid

#61539

Purpose

Expresses mir124a in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

mir124a (MIR124-1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pLX304 GDF11-V5 blast

Plasmid

#83098

Purpose

Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

GDF11 (GDF11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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CFP-FRBx5

Plasmid

#103774

Purpose

expresses CFP-FRBx5 protein in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

CFP-FRBx5

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-FKBPx5

Plasmid

#103775

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-FKBPx5

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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HsCLYBL(D320A)-FLAG

Plasmid

#111291

Purpose

mammalian expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL D320A (CLYBL Human)

Tags

linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

Expression

Mammalian

Mutation

D320A

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadCBEa

Plasmid

#193844

Purpose

Express TadCBEa (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

SaCas9 TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PLAGL1

Plasmid

#61542

Purpose

Expresses PLAGL1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

PLAGL1 (PLAGL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only