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169,450 results

Showing: 2681 - 2720 of 169450 results

  8. pcDNA3-N-HA-NEK7 K64M

    Plasmid
    #75143
    Purpose
    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells
    Depositor
    Bruce Beutler
    Article
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lys 64 mutated to Met; kinase inactive and F236L …
    Promoter
    CMV
    Available Since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    75143-plasmid-map-sequence-id-147264

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  9. hKv2.1-pEGFP-C1

    Plasmid
    #111538
    Purpose
    Expresses human KCNB1 in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human Kv2.1 (KCNB1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111538-plasmid-map-sequence-id-217177

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  11. pLV-Blast_hCOL4A5

    Plasmid
    #198100
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198100-plasmid-map-sequence-id-389263

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  16. FLAG-hPAX3 (#395)

    Plasmid
    #78335
    Purpose
    Overexpression of human PAX3 ORF with a N-terminal FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    PAX3 (PAX3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

    Plasmid
    #128450
    Purpose
    lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128450-plasmid-map-sequence-id-256464

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  24. pcDNA3.1-C-Luc

    Plasmid
    #185358
    Purpose
    For mammalian expression of C-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 398-550).
    Depositor
    Ayaz Najafov
    Insert
    Firefly Luciferase
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    185358-plasmid-map-sequence-id-376433

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  26. pPICZc-ScAct1*-thymosinB-8His

    Plasmid
    #111148
    Purpose
    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    Act1 (ACT1 Budding Yeast)
    Use
    Pichia pastoris integration plasmid
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Promoter
    AOX1
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111148-plasmid-map-sequence-id-214055

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  30. Lenti-pHSE::Cre-mRuby3

    Plasmid
    #164136
    Purpose
    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction
    Depositor
    Hyongbum Kim
    Article
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Insert
    HSE promoter-Cre-T2A-mRuby3
    Use
    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…
    Expression
    Mammalian
    Promoter
    HSE synthetic promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    164136-plasmid-map-sequence-id-323779

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  35. pKEK3238

    Plasmid
    #234092
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234092-plasmid-map-sequence-id-467988

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  37. mCit-APPAPAPPAP-gs

    Plasmid
    #228091
    Purpose
    Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, APPAPAPPAP
    Expression
    Mammalian
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228091-plasmid-map-sequence-id-451163

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Showing: 2681 - 2720 of 169450 results