We narrowed to 11,910 results for: 112

1. PLIN2-V5-APEX2

   Plasmid

   #170573

   Purpose

   A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

   Depositor

   James Olzmann

   Article

   Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

   Insert

   PLIN2 (Plin2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5-APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ELONGIN BC (XX01TCEB1A-c001)

   Plasmid

   #110274

   Purpose

   Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C

   Depositor

   Nicola Burgess-Brown

   Insert

   Elongin BC (ELOC, ELOB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Elongin B is full length. Elongin C contains a.a…

   Promoter

   T7

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. plx208-CMV-Gal4DBD-CapN-SsrA-CapC

   Plasmid

   #213533

   Purpose

   Expresses one of the split transcription factor component in mammalian cells

   Depositor

   Wenjing Wang

   Article

   Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

   Insert

   SspB-EGFP-VP16
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJA181

   Plasmid

   #128539

   Purpose

   Expresses anti-Prunus dulcis scFv Ab+BirA tag

   Depositor

   Marcos Alcocer

   Article

   de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

   Insert

   scFv Ab clone PD1F6 plus BirA tag
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-bSV-831-1281

   Plasmid

   #40962

   Depositor

   Elizabeth Luna

   Article

   Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

   Insert

   supervillin (SVIL Bovine)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-830 and 1282-1792

   Promoter

   Available since

   Dec. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

6. plx208-CMV-SspB-EGFP-VP16

   Plasmid

   #213532

   Purpose

   Expresses one of the split transcription factor component in mammalian cells

   Depositor

   Wenjing Wang

   Article

   Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

   Insert

   SspB-EGFP-VP16
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCML 313

   Plasmid

   #98586

   Purpose

   pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Insert

   csrB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCML 314

   Plasmid

   #98584

   Purpose

   pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Inserts

   CsrA
   csrB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATGL*-V5-APEX2

   Plasmid

   #170574

   Purpose

   A C-terminal APEX2 fusion to the human ATGL S47A protein

   Depositor

   James Olzmann

   Article

   Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

   Insert

   ATGL* (PNPLA2 Human)
   

   Use

   Lentiviral

   Tags

   V5-APEX2

   Expression

   Mammalian

   Mutation

   changed serine 47 to alanine

   Promoter

   CMV

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNLS-2xBFP(4C)

    Plasmid

    #176151

    Purpose

    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    NLS-2xBFP(4C)
    

    Use

    Tags

    6xHis tag and SV40 nuclear localization sequence …

    Expression

    Bacterial

    Mutation

    Compared with EGFP, the two BFP domains have the …

    Promoter

    Ptrc

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM9-BetaGal-8C

    Plasmid

    #172489

    Purpose

    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    M9-BetaGal-8C
    

    Use

    Tags

    6xHis and M9 nuclear localization sequence (from …

    Expression

    Bacterial

    Mutation

    BetaGal has two cysteine mutations (C77A, C1022S)…

    Promoter

    T7

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAmCyan1-C1-ADCY6

    Plasmid

    #113905

    Purpose

    N-terminal Cyan Fluorescent tag in Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 (ADCY6 Human)
    

    Use

    Tags

    Cyan

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. plx208-CMV-Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit

    Plasmid

    #213538

    Purpose

    Expresses the CapC-caged opioid peptide in mammalian cells

    Depositor

    Wenjing Wang

    Article

    Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

    Insert

    Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCas9-beta-mmr

    Plasmid

    #169241

    Purpose

    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    mutL-E36K
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed glutamic acid 36 to lysine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. shMEK1/2 puro

    Plasmid

    #72567

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. shMEK1/2 v2 neo

    Plasmid

    #72570

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. shMEK1/2 v2 puro

    Plasmid

    #72568

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-bSV1_1-1009

    Plasmid

    #129471

    Purpose

    Mammalian expression of the N-terminus + central domain of bovine SV1

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    Supervillin (SVIL Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 1-1009

    Promoter

    CMV

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. shMEK1/2 neo

    Plasmid

    #72569

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCTCON2-Aga2p-Flag-CapN-TEVcs-HA

    Plasmid

    #213530

    Purpose

    Expresses CapN-caged TEVcs on yeast surface

    Depositor

    Wenjing Wang

    Article

    Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

    Insert

    Aga2p-Flag-CapN-TEVcs-HA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1/10

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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