We narrowed to 7,282 results for: Ank

1. pAC026

   Plasmid

   #227598

   Purpose

   Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHM6-Q74

   Plasmid

   #40264

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q74 (HTT Human)
   

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR221-mCherry

   Plasmid

   #79509

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   mCherry RFP
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTF-lacZ-rfp

   Plasmid

   #153037

   Purpose

   CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #126218

   Purpose

   Global labeling of excitatory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CamKIIa

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGFP FAK

   Plasmid

   #50515

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Article

   Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

   Insert

   protein tyrosine kinase
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETCON-PTX-S1-220

   Plasmid

   #67747

   Purpose

   Pertussis Toxin Subunit 1 Yeast Display Plasmid

   Depositor

   Timothy Whitehead

   Article

   Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

   Insert

   PTxS1-220
   

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIPZ TREX1-KI

   Plasmid

   #127701

   Purpose

   For knocking in TREX1 in mouse - Doxycyclin inducible

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   mouse TREX1 cDNA (Trex1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-mRuby2-Gephyrin.FingR-IL2RGTC

   Plasmid

   #125696

   Purpose

   Global labeling of inhibitory synapses (red fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   mRuby2-Gephyrin.FingR-IL2RGTC
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDA083

    Plasmid

    #131389

    Purpose

    eGFP expression

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    eGFP
    

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTF-lacZ

    Plasmid

    #153036

    Purpose

    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDA007

    Plasmid

    #131380

    Purpose

    ORFeus expression, WT

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus-Hs
    

    Tags

    ORF2-3xFlag

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET30b_OmpAT7Sav

    Plasmid

    #138589

    Purpose

    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

    Insert

    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    

    Use

    Synthetic Biology

    Tags

    OmpA export signal and T7 tag

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC0.419

    Plasmid

    #203935

    Purpose

    Down Flank. Neutral site. Down flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901.

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Insert

    aquI DOWN
    

    Use

    Synthetic Biology

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Beclin1

    Plasmid

    #21150

    Depositor

    Junying Yuan

    Article

    Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

    Insert

    PcDNA3-Beclin1 (BECN1 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0045-EF1a-PguCas13b-NES-HIV

    Plasmid

    #103861

    Purpose

    Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PguCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p{CFD4-3xP3::DsRed}

    Plasmid

    #86864

    Purpose

    Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDA056

    Plasmid

    #131387

    Purpose

    ORFeus expression, WT

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus-Hs
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbS8c-ddcpf1-rfp

    Plasmid

    #153038

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Expression

    Bacterial

    Mutation

    E993A

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. tdTomato-LacI

    Plasmid

    #183921

    Purpose

    Lac repressor labeled with tdTomato; can be used to label lacO sites

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdTomato-LacI (lacI Synthetic)
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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