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Addgene
Showing: 1341 - 1360 of 8934 results

  1. pBI-MCS-EYFP

    Plasmid
    #58855
    Purpose
    The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    enhanced yellow fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58855-plasmid-map-sequence-id-98989

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  3. pVP073

    Plasmid
    #222076
    Purpose
    eforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222076-plasmid-map-sequence-id-440974

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  4. pVP074

    Plasmid
    #222077
    Purpose
    eforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222077-plasmid-map-sequence-id-440969

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  5. pVP075

    Plasmid
    #222078
    Purpose
    AeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222078-plasmid-map-sequence-id-440967

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  7. ttAtCas12a+int

    Plasmid
    #182384
    Purpose
    Gives very high editing efficiency in barley. Also works in Brassica oleracea
    Depositor
    Wendy Harwood
    Article
    Lawrenson et al bioRxiv 2022.04.28.489853
    Insert
    LbCas12a coding sequence with D156R and Arabidopsis introns
    Use
    CRISPR
    Tags
    Expression
    Mutation
    D156R + 8 Arabidopsis introns added
    Promoter
    None
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182384-plasmid-map-sequence-id-361472

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  8. PX854

    Plasmid
    #62886
    Purpose
    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa573-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62886-plasmid-map-sequence-id-105909

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  9. T777T

    Plasmid
    #113082
    Purpose
    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113082-plasmid-map-sequence-id-218958

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  17. tTA_BFP

    Plasmid
    #58854
    Purpose
    Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    tTA transcription factor fused to enhanced blue fluorescent protein
    Use
    Tags
    BFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58854-plasmid-map-sequence-id-327655

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Showing: 1341 - 1360 of 8934 results