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We narrowed to 16,144 results for: LEA

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Showing: 1341 - 1360 of 16144 results

  1. pNUT N6His K206E/K534A hTFNG

    Plasmid
    #70133
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.
    Insert
    N6His K206e/K534A hTFNG (TF Human)
    Tags
    N-terminal signal peptide, 4 aa link, 6 His, Fact…
    Expression
    Mammalian
    Mutation
    changed Lys206 to a glutamic acid and Lys534 to a…
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70133-plasmid-map-sequence-id-129418

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  3. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  4. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid
    #79886
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Ubiquitin
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79886-plasmid-map-sequence-id-150062

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  12. 3xEGFP-mMeikin(1-391, E156R, R159E) - hRad21(142-275) - mMeikin(387-434)

    Plasmid
    #174712
    Purpose
    Mammalian expression of mouse Meikin with wild-type Separase cleavage site eliminated and C-terminal Separase cleavage site inserted
    Depositor
    Iain Cheeseman
    Article
    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8
    Insert
    Meikin (Meikin Mouse)
    Tags
    3xEGFP
    Expression
    Mammalian
    Mutation
    mMeikin: 1-391, E156R, R159E; hRad21: 142-275; mM…
    Available Since
    Oct. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174712-plasmid-map-sequence-id-343365

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  15. pcDNA3.1(+)-AKAP95-FRB

    Plasmid
    #138254
    Purpose
    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    AKAP95-FRB (AKAP8 Human)
    Tags
    FRB rapamycin binding domain and HA epitope
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    138254-plasmid-map-sequence-id-307498

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  17. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid
    #92120
    Purpose
    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    MS2-P65-HSF1 (HSF1 Human, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92120-plasmid-map-sequence-id-197614

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  18. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid
    #182424
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    Tags
    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182424-plasmid-map-sequence-id-360472

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  20. NLS-4LNDD-pcDNA3.1(+)

    Plasmid
    #172313
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells
    Depositor
    Joshua Rosenthal
    Article
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172313-plasmid-map-sequence-id-339585

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Showing: 1341 - 1360 of 16144 results