We narrowed to 29,593 results for: PLE;

1. pLentiCrisprV2 sgRNA hCRY1 c-term

   Plasmid

   #179453

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human CRY1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-CMV-puro_FlnaABD-TS-16flag

   Plasmid

   #234868

   Purpose

   Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

   Depositor

   Michael Murrell

   Article

   Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

   Insert

   FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16
   

   Use

   Lentiviral

   Tags

   GFP, RFP, FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

   Plasmid

   #234869

   Purpose

   Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.

   Depositor

   Michael Murrell

   Article

   Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

   Insert

   FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
   

   Use

   Lentiviral

   Tags

   GFP, RFP, FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti CMV SNAP MDC1

   Plasmid

   #227375

   Purpose

   SNAP–MDC1 expression

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231364

   Purpose

   Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-mCherry-CAAX PuroR

   Plasmid

   #166228

   Purpose

   Lentiviral plasmid with Puromycin selection for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   mCherry-CAAX
   

   Use

   Lentiviral

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti Puro HA-Ubiquitin-K48

   Plasmid

   #236290

   Purpose

   Lentiviral vector expressing HA-tagged ubiquitin K48 mutant

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Ubiquitin K48 (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Single ubiquitin coding sequence containing K48 m…

   Promoter

   CMV

   Available since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pLenti Puro HA-Ubiquitin-K63

   Plasmid

   #236291

   Purpose

   Lentiviral vector expressing HA-tagged ubiquitin K63 mutant

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Ubiquitin K63 (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Single ubiquitin coding sequence containing K63 m…

   Promoter

   CMV

   Available since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pLenti-STAT3-linker-TagGFP2

   Plasmid

   #121427

   Purpose

   WT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   STAT3-linker-TagGFP2
   

   Use

   Tags

   TagGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-Mito-Car-GECO

    Plasmid

    #100765

    Purpose

    Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Lenti-Mito-Car-GECO
    

    Use

    Lentiviral

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti X2 Hygro DEST (w17-1)

    Plasmid

    #17295

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Hygro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-X1-Neo-GFP-ATL3

    Plasmid

    #109024

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti Lifeact-mRuby2 BlastR

    Plasmid

    #84384

    Purpose

    Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-CMV-Blast-DNTGFBR2-HA

    Plasmid

    #130888

    Purpose

    Expresses dominant negative mutant human TGFBR2

    Depositor

    Gian-Paolo Dotto

    Article

    Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

    Insert

    TGFBR2 (TGFBR2 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Dominant Negative receptor lacking cytosolic cata…

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid

    #55200

    Purpose

    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-5HRE-GFP NeoR

    Plasmid

    #128960

    Purpose

    Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

    Depositor

    Carlos Carmona-Fontaine

    Article

    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

    Insert

    5X HRE of VEGF (VEGFA Human)
    

    Use

    Lentiviral

    Tags

    d2EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-EF-FH-TAZ-ires-blast

    Plasmid

    #52083

    Purpose

    Lentiviral expression vector for TAZ with N-terminal FLAG and His tags

    Depositor

    Yutaka Hata

    Article

    Yang et al Mol Cell Biol. 2014 Feb 18.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLentiPGK Puro DEST JNKKTRmCerulean3

    Plasmid

    #90232

    Purpose

    Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-dCas9-T2A-GFP

    Plasmid

    #193781

    Purpose

    Tricistronic expression of dCas9-EGFP-BSD

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    dead Cas9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with existing Cas9 promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-H3.3(WT)-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225701

    Purpose

    Lentiviral expression of AID degron tagged Wildtype H3.3 and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (WT) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral

    Tags

    3xAID HA and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge